Overview

  • Product name
    Anti-Lipid A antibody [26-5]
    See all Lipid A primary antibodies
  • Description
    Mouse monoclonal [26-5] to Lipid A
  • Tested applications
    Suitable for: Agglutination, ELISA, WBmore details
  • Immunogen

    Synthetic Lipid A.

  • General notes


    Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is sterile filtered.
  • Primary antibody notes
    Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria.
  • Clonality
    Monoclonal
  • Clone number
    26-5
  • Myeloma
    unknown
  • Isotype
    IgG2b
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration. Use NEAT.
ELISA 1/10.

in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.

WB Use at an assay dependent concentration. PubMed: 21873161

Target

  • Relevance
    Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
  • Cellular localization
    Secreted

References

This product has been referenced in:
  • Martin I  et al. Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo. J Immunol 194:3924-36 (2015). Read more (PubMed: 25780044) »
  • Kadowaki T  et al. Functional Characterization of Lipopolysaccharide derived from Symbiotic Bacteria in Rice as a Macrophage-activating Substance. Anticancer Res 31:2467-76 (2011). WB . Read more (PubMed: 21873161) »

See all 2 Publications for this product

Customer reviews and Q&As

We do not have in-house data demonstrating relative specificity of antibody ab8467 for lipid A versus IVa. The following reference discusses this antibody, clone 26-5, but I do not know if it will help answer your question, as we do not have access to ...

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Thank you for contacting us.
This antibody is supplied at a concentration of 0.2 mg/mL and each vial contains 200ug of antibody. For ELISA, we recommend using this antibody at a dilution of 1:10 for ELISA and 1:200 for WB. These dilutions will nee...

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Thank you for your enquiry. Unfortunately, we do not know which region this product will detect but we know that it reacts with the hydrophilic part of Lipid-A. I am afraid that the peptide sequence has not been mapped.

There is no specific reason for using tapwater. You can also use Milli-Q, but we did not test this antibody on Western blot.

Thank you for your enquiry. Unfortunately, the only anti-lipid A antibody which we currently carry is ab8467. To see if another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World...

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Due to a recent customer enquiry our understanding now is that this antibody reacts with the hydrophilic region of synthetic lipid A (with no sugars attached) and not with Re-LPS (which add KDO sugars to the hydrophilic region of lipid A and block the ...

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Unfortunately, we do not have any record of this Lipopolysaccharide antibody being tested on the E.Coli K-12 strain.

Just to follow on from our phone call. I would expect this antibody to work in IC as it works in rough bacterial preparations, although it has only been tested in ELISA. However, we have a policy of not providing samples sizes, as we find it takes ...

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The immunogen for ab8467 was the Re mutant of Salmonella typhimurium. The antibody recognises the lipid A/KDO region of LPS

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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