Overview

  • Product nameAnti-Lipid A antibody
    See all Lipid A primary antibodies
  • Description
    Goat polyclonal to Lipid A
  • SpecificityThe antibody was tested in direct IF on cells fixed with acetone and the secondary antibody was labeled with FITC.
  • Tested applicationsSuitable for: Conjugation, ICC/IFmore details
  • Species reactivity
    Reactive with numerous members of the Enterobacteriaciae. Pseudomonas aeruginosa, Klebsiella pneumoniae, E. coli O157, Salmonella enteriditis, Enterobacter aerogenes, E. hermanii, Yersinia enterocolitica and Shigella sonnei. Not yet tested in other species.
  • Immunogen

    Whole cells prep of Lipid A from E. coli O157.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesSodium sulfate precipitation and ion-exchange chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution. Fix with acetone.

Target

  • RelevanceLipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
  • Cellular localizationSecreted

References for Anti-Lipid A antibody (ab20001)

This product has been referenced in:
  • Miyata ST  et al. Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun 79:2941-9 (2011). Read more (PubMed: 21555399) »

See 1 Publication for this product

Product Wall

Application ELISA
Sample Mouse Serum (also plasma)
Specification also plasma
Type Other
Blocking step Pierce protein free buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Jan 14 2014

Abreviews
Application Western blot
Sample Acinetobacter baumannii Cell lysate - whole cell (bacteria)
Loading amount 300000 cells
Specification bacteria
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.3% · Temperature: 20°C
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Submitted Jan 09 2012

Abreviews
Application Western blot
Sample Pseudomonas aeruginosa Cell lysate - whole cell (bacteria)
Loading amount 300000 cells
Specification bacteria
Gel Running Conditions Non-reduced Denaturing (gel 12%)
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.3% · Temperature: 20°C
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Submitted Jan 05 2012

Code de remise : XXX%XXX-XXXX Date d’expiration : JJ MM AAAA Je suis ravi que vous souhaitiez participer à notre offre et tester ab20001 chez Acinetobacter baumannii. Le code de réduction correspondant est 100%ABR-E7TFB. Ce code de réduction ...

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Thank you for contacting us. Lab let me know that an affinity constant of Catalog ab20001, Goat anti Lipid A, has not been determined. This antibody recognizes the lipid A portion of LPS . When tested by IFA, the antibody shows strongest reac...

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Thank you for your enquiry. The antibody was tested in direct IF on cells fixed with acetone and the secondary antibody was labeled with FITC. I have added this information to the online datasheet. I hope this information helps, please do not he...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"