Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970)


  • Product name
    Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)
  • Detection method
  • Sample type
    Plasma, Cell culture extracts, Tissue Extracts
  • Assay type
  • Sensitivity
    > 0.1 nmol/well
  • Product overview

    Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970) provides a convenient tool for sensitive detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

    Visit our FAQs page for tips and troubleshooting.

    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Notes

    Lipid peroxidation refers to the oxidative degradation of lipids. In this process free radicals take electrons from the lipids (generally in cell membranes), resulting in cell damage. Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform



Our Abpromise guarantee covers the use of ab118970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.


  • 10 mg of tissue were homogenized on ice in 300 μL of MDA lysis buffer, then centrifuged (13,000 × g, 10 min) to remove insoluble materials. 10 μL of plasma were mixed with 500 μL of 42 mM H2SO4 and 125 μL of phosphotungstic acid solution at room temperature for 5 min. After centrifuging (13,000 × g, 3 min), the pellet was re-suspended on ice with 100 μL of double-distilled H2O. Then, 200 μL of solution and 600 μL of 2-thiobarbituric acid solution were incubated at 95°C for 60 min, before cooling to room temperature in the ice bath for 10 min. The intensity of absorbance at 532 nm was proportional to the MDA level.

  • Typical MDA standard calibration curve using colorimetric reading.

  • Typical MDA standard calibration curve using fluorometric reading.

  • Measurement of MDA in human plasma (20 µl) and rat liver lysate (10 mg).



This product has been referenced in:
  • Ertan NZ  et al. Alterations of erythrocyte rheology and cellular susceptibility in end stage renal disease: Effects of peritoneal dialysis. PLoS One 12:e0171371 (2017). Read more (PubMed: 28158274) »
  • Long YM  et al. Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli. Int J Nanomedicine 12:3193-3206 (2017). Read more (PubMed: 28458540) »

See all 22 Publications for this product

Customer reviews and Q&As

I confirmed with the laboratories that typically we do not recommend RIPA buffer for these kits since it contains SDS which can denature proteins.
We suggest to use the lysis buffer that comes with the ab118970 Lipid Peroxidation (MDA) Assay ...

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Yes, heparin should be fine to use with ab65328 and ab118970.

Below please find answers to your questions:

A1: We have found that the 10 ul plasma worked well. How much more plasma is used depends on the protein content.
A2: If there is low amounts of MDA adduct formed in plasma samples, then the flu...

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I double checked and unfortunately, have confirm that we do not have a kit for measuring NADPH oxidase in our catalog at the moment. We do offer ab65349 which is a NADP/NADPH Assay Kit:

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Centrifuge the samples and take only the clear supernatant for the next step.

Thank you for your enquiry.

I can confirm that you will need to use the n-butanol for the standards as well, since it helps in isolating the MDA-TBA adduct.

I hope this information has been useful for you. Please do not hesitate t...

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Thank you for contacting us about this issue. The lab says the TBA generally goes into solution after mixing the TBA in acetic acid with the water. Did you see any apparent dissolving after mixing the two by vortexing? Did you try heating it in a fume ...

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Regarding ab133085, we do not have data for the collection, freezing and storage of plant tissue samples for 6 months at -80ºC. From your customer’s protocol, they did a good job of collecting, freezing and storing their tissues, but th...

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Thank you for your inquiry.

I heard from the lab that you can start with a greater number of cells and homogenize them in the same 300 ul MDA lysis buffer. They didn't think deproteinization would help. But you may want to use n-butanol to ex...

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Yes, that is correct: 0, 2, 4, 6, 8, 10 nmol per well, since you have used half the suggested standard volume. Reporting nmol/mg should be fine, but converting to ng/mg is easy enough to do. I am not sure why the other kit we discussed uses uM units bu...

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1-10 of 29 Abreviews or Q&A


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