Application notesThis antibody gave a positive result in ELISA against the immunizing peptide (ab53547).
Antibody detection limit dilution 1/23000
Western Blot: Preliminary experiments gave bands at approx 50kDa and 26kDa in Human Adipose lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted size of 98.7kDa. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Not yet tested in other applications.
FunctionPlays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
Tissue specificityAbundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.
Involvement in diseaseDefects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.
Sequence similaritiesBelongs to the lipin family.
DomainContains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA. Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
Post-translational modificationsPhosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid. Sumoylated.
Marker DF et al. The new small-molecule mixed-lineage kinase 3 inhibitor URMC-099 is neuroprotective and anti-inflammatory in models of human immunodeficiency virus-associated neurocognitive disorders. J Neurosci33:9998-10010 (2013).
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