Overview

  • Product name
  • Description
    Rabbit polyclonal to Lipin 1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human Lipin 1.

    (Peptide available as ab86631.)

  • Positive control
    • This antibody gave a positive signal in the following Mouse Tissue Lysates: Liver; Pancreas, Kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab70138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

Target

  • Function
    Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
  • Tissue specificity
    Abundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.
  • Involvement in disease
    Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.
  • Sequence similarities
    Belongs to the lipin family.
  • Domain
    Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
    Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
  • Post-translational
    modifications
    Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
    Sumoylated.
  • Cellular localization
    Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC=3.1.3.4 antibody
    • KIAA0188 antibody
    • Lipin-1 antibody
    • Lpin1 antibody
    • LPIN1_HUMAN antibody
    • PAP1 antibody
    • Phosphatidate phosphatase LPIN1 antibody
    see all

Images

  • All lanes : Anti-Lipin 1 antibody (ab70138) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Cai K  et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014). WB . Read more (PubMed: 24369117) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Airway epithelial cells)
Specification
Airway epithelial cells
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Prof. Marc Chanson

Verified customer

Submitted Sep 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Loading amount
30 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
50 µg
Specification
mouse heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Apr 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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