The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 3 µg/ml.
FunctionRegulates fatty acid metabolism. Magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis.
Tissue specificitySignificant expression in intestine and other regions of the gastrointestinal tract.
Sequence similaritiesBelongs to the lipin family.
DomainContains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif known to be essential for phosphatidate phosphatase activity. Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL) motif, a motif known to be a transcriptional binding motif.
ab53548 at 2.5µg/ml staining Lipin 3 in human thyroid gland tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used for detection.
References for Anti-Lipin 3 antibody (ab53548)
has not yet been referenced specifically in any publications.
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