Overview

  • Product nameAnti-Lipin 3 antibody
    See all Lipin 3 primary antibodies
  • Description
    Rabbit polyclonal to Lipin 3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Lipin 3.

  • Positive control
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionRegulates fatty acid metabolism. Magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis.
  • Tissue specificitySignificant expression in intestine and other regions of the gastrointestinal tract.
  • Sequence similaritiesBelongs to the lipin family.
  • DomainContains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif known to be essential for phosphatidate phosphatase activity.
    Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL) motif, a motif known to be a transcriptional binding motif.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ450M14.2 antibody
    • dJ450M14.3 antibody
    • dJ620E11.2 antibody
    • Lipin 3 like antibody
    • Lipin-3 antibody
    • Lipin-3-like antibody
    • Lipin3 antibody
    • LIPN3L antibody
    • LPIN 3 antibody
    • Lpin3 antibody
    • LPIN3_HUMAN antibody
    • Phosphatidate phosphatase LPIN3 antibody
    • RP4 620E11.3 antibody
    • SMP2 antibody
    see all

Anti-Lipin 3 antibody images

  • Anti-Lipin 3 antibody (ab70159) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 94 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,45 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab70159 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70159, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Lipin 3 antibody (ab70159)

ab70159 has not yet been referenced specifically in any publications.

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