Overview

  • Product name
    Anti-Lipoamide Dehydrogenase antibody
    See all Lipoamide Dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Lipoamide Dehydrogenase
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a sequence at the C-terminal of Human Lipoamide Dehydrogenase.

  • Positive control
    • Human Breast cancer tissue; Rat brain and liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab119422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

Target

  • Function
    Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
  • Involvement in disease
    Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
    Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
  • Sequence similarities
    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dehydrogenase complex, E3 component antibody
    • Diaphorase antibody
    • Dihydrolipoamide dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase mitochondrial antibody
    • dld antibody
    • DLDD antibody
    • DLDH antibody
    • DLDH_HUMAN antibody
    • E3 antibody
    • E3 branched chain aplha-keto acid antibody
    • E3 component of pyruvate dehydrogenase antibody
    • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
    • GCSL antibody
    • Glycine cleavage system L protein antibody
    • Glycine cleavage system protein L antibody
    • LAD antibody
    • lipoamide dehydrogenase antibody
    • Lipoamide reductase antibody
    • Lipoyl dehydrogenase antibody
    • mitochondrial antibody
    • OTTHUMP00000206744 antibody
    • OTTHUMP00000206746 antibody
    • OTTHUMP00000206748 antibody
    • OTTHUMP00000206749 antibody
    • PHE 3 antibody
    • PHE3 antibody
    • Pyruvate dehydrogenase component E3 antibody
    see all

Images

  • All lanes : Anti-Lipoamide Dehydrogenase antibody (ab119422) at 1 µg/ml

    Lane 1 : Rat Brain tissue lysate
    Lane 2 : Rat Liver tissue lysate


    Predicted band size : 54 kDa
  • ab119422 at 1µg/ml staining Lipoamide Dehydrogenase in by Paraffin-embedded Human Breast cancer tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Arrabal S  et al. Pharmacological Blockade of Cannabinoid CB1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle. PLoS One 10:e0145244 (2015). Read more (PubMed: 26671069) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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