Anti-Lipoamide Dehydrogenase antibody (ab152105)

Overview

  • Product name
    Anti-Lipoamide Dehydrogenase antibody
    See all Lipoamide Dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Lipoamide Dehydrogenase
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 264-469 of Human DLD (Uniprot: P09622).

  • Positive control
    • 293T and Mmouse brain whole cell lysate, Hep3B cells, Human Gastric CA tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab152105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
  • Involvement in disease
    Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
    Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
  • Sequence similarities
    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dehydrogenase complex, E3 component antibody
    • Diaphorase antibody
    • Dihydrolipoamide dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase mitochondrial antibody
    • dld antibody
    • DLDD antibody
    • DLDH antibody
    • DLDH_HUMAN antibody
    • E3 antibody
    • E3 branched chain aplha-keto acid antibody
    • E3 component of pyruvate dehydrogenase antibody
    • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
    • GCSL antibody
    • Glycine cleavage system L protein antibody
    • Glycine cleavage system protein L antibody
    • LAD antibody
    • lipoamide dehydrogenase antibody
    • Lipoamide reductase antibody
    • Lipoyl dehydrogenase antibody
    • mitochondrial antibody
    • OTTHUMP00000206744 antibody
    • OTTHUMP00000206746 antibody
    • OTTHUMP00000206748 antibody
    • OTTHUMP00000206749 antibody
    • PHE 3 antibody
    • PHE3 antibody
    • Pyruvate dehydrogenase component E3 antibody
    see all

Images

  • Anti-Lipoamide Dehydrogenase antibody (ab152105) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 54 kDa
  • Anti-Lipoamide Dehydrogenase antibody (ab152105) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 54 kDa
  • Immunofluorescence analysis of methanol-fixed Hep3B cells labeling Lipoamide Dehydrogenase using ab152105 at a 1/100 dilution. Bottom image is costained with hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human gastric CA tissue labeling Lipoamide Dehydrogenase using ab152105 at a 1/100 dilution.

References

ab152105 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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