Anti-Lipoamide Dehydrogenase antibody (ab85930)


  • Product nameAnti-Lipoamide Dehydrogenase antibody
    See all Lipoamide Dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Lipoamide Dehydrogenase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 460 - 509 (AGEMVNEAAL ALEYGASCED IARVCHAHPT LSEAFREANL AASFGKSINF) of Human Lipoamide Dehydrogenase (NP_000099)

  • Positive control
    • Jurkat cell lysate



Our Abpromise guarantee covers the use of ab85930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.


  • FunctionLipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
  • Involvement in diseaseNote=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
    Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
  • Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Post-translational
    Tyrosine phosphorylated.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dehydrogenase complex, E3 component antibody
    • Diaphorase antibody
    • Dihydrolipoamide dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase antibody
    • Dihydrolipoyl dehydrogenase mitochondrial antibody
    • dld antibody
    • DLDD antibody
    • DLDH antibody
    • DLDH_HUMAN antibody
    • E3 antibody
    • E3 branched chain aplha-keto acid antibody
    • E3 component of pyruvate dehydrogenase antibody
    • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
    • GCSL antibody
    • Glycine cleavage system L protein antibody
    • Glycine cleavage system protein L antibody
    • LAD antibody
    • lipoamide dehydrogenase antibody
    • Lipoamide reductase antibody
    • Lipoyl dehydrogenase antibody
    • mitochondrial antibody
    • OTTHUMP00000206744 antibody
    • OTTHUMP00000206746 antibody
    • OTTHUMP00000206748 antibody
    • OTTHUMP00000206749 antibody
    • PHE 3 antibody
    • PHE3 antibody
    • Pyruvate dehydrogenase component E3 antibody
    see all

Anti-Lipoamide Dehydrogenase antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Lipoamide Dehydrogenase with ab85930 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Lipoamide Dehydrogenase. Right - Merge.
  • Anti-Lipoamide Dehydrogenase antibody (ab85930) at 1 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Gel concentration 12%

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
  • Anti-Lipoamide Dehydrogenase antibody (ab85930) at 1/1000 dilution + rat proximal kidney tubules purified from the cortex at 1 µg/ml

    Predicted band size : 54 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)

References for Anti-Lipoamide Dehydrogenase antibody (ab85930)

ab85930 has not yet been referenced specifically in any publications.

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