Recombinant
RabMAb

Anti-Lipoamide Dehydrogenase antibody [EPR6634(B)] (ab124926)

Overview

  • Product name
    Anti-Lipoamide Dehydrogenase antibody [EPR6634(B)]
    See all Lipoamide Dehydrogenase primary antibodies
  • Description
    Rabbit monoclonal [EPR6634(B)] to Lipoamide Dehydrogenase
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Lipoamide Dehydrogenase.

  • Positive control
    • Jurkat, MCF7, HeLa, and 293T cell lysates; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
    • Involvement in disease
      Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
      Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
    • Sequence similarities
      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    • Post-translational
      modifications
      Tyrosine phosphorylated.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dehydrogenase complex, E3 component antibody
      • Diaphorase antibody
      • Dihydrolipoamide dehydrogenase antibody
      • Dihydrolipoyl dehydrogenase antibody
      • Dihydrolipoyl dehydrogenase mitochondrial antibody
      • dld antibody
      • DLDD antibody
      • DLDH antibody
      • DLDH_HUMAN antibody
      • E3 antibody
      • E3 branched chain aplha-keto acid antibody
      • E3 component of pyruvate dehydrogenase antibody
      • E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody
      • GCSL antibody
      • Glycine cleavage system L protein antibody
      • Glycine cleavage system protein L antibody
      • LAD antibody
      • lipoamide dehydrogenase antibody
      • Lipoamide reductase antibody
      • Lipoyl dehydrogenase antibody
      • mitochondrial antibody
      • OTTHUMP00000206744 antibody
      • OTTHUMP00000206746 antibody
      • OTTHUMP00000206748 antibody
      • OTTHUMP00000206749 antibody
      • PHE 3 antibody
      • PHE3 antibody
      • Pyruvate dehydrogenase component E3 antibody
      see all

    Images

    • ab124926 at 1/100 dilution staining Lipoamide Dehydrogenase in Paraffin-embedded Human colon tissue by Immunohistochemistry.
    • All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6634(B)] (ab124926) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 54 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124926 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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