Overview

  • Product name
    Anti-Lipocalin-2 / NGAL antibody
    See all Lipocalin-2 / NGAL primary antibodies
  • Description
    Goat polyclonal to Lipocalin-2 / NGAL
  • Specificity
    Lipocalin-2 / NGAL
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Lipocalin-2/ NGAL aa 60-72. Synthetic peptide:

    C-AILREDKDPQKMY

    , corresponding to amino acids 60-72 of Human Lipocalin 2/ NGAL .
    Sequence:

    C-AILREDKDPQKMY

  • Positive control
    • Human kidney lysate. Human spleen tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab31289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 2 - 3 µg/ml.

Target

  • Function
    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificity
    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization
    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • Database links
  • Alternative names
    • 24p3 antibody
    • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
    • Alpha 2 microglobulin related protein antibody
    • HGNC:6526 antibody
    • HNL antibody
    • Lcn 2 antibody
    • Lcn2 antibody
    • Lipocalin-2 antibody
    • Migration stimulating factor inhibitor antibody
    • MSFI antibody
    • Neutrophil gelatinase associated lipocalin antibody
    • Neutrophil gelatinase associated lipocalin precursor antibody
    • Neutrophil gelatinase-associated lipocalin antibody
    • NGAL antibody
    • NGAL_HUMAN antibody
    • Oncogene 24p3 antibody
    • Oncogenic lipocalin 24p3 antibody
    • p25 antibody
    • Siderocalin antibody
    • siderocalin LCN2 antibody
    • SV40 induced 24P3 protein antibody
    • Uterocalin antibody
    see all

Images

  • ab31289 at 2.5µg/ml staining Lipocalin 2 / NGAL in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, AP-staining.

     

  • Primary: ab31289, 0.03 ug/mL Sample: Human kidney lysate (35ug protein in RIPA buffer)

    Predicted band size : 23 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References

ab31289 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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