Overview

  • Product nameAnti-Lipocalin-2 / NGAL antibody
    See all Lipocalin-2 / NGAL primary antibodies
  • Description
    Rabbit polyclonal to Lipocalin-2 / NGAL
  • SpecificityLipocalin-2 / NGAL
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Lipocalin-2/ NGAL (N terminal). Synthetic peptide derived from near the N terminus of Human Lipocalin 2/ NGAL.

  • Positive control
    • Human stomach adenocarcinoma and breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab93963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 for 30 minutes at RT. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
    • Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
    • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
    • Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
    • Information by UniProt
    • Database links
    • Alternative names
      • 24p3 antibody
      • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
      • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
      • Alpha 2 microglobulin related protein antibody
      • HGNC:6526 antibody
      • HNL antibody
      • Lcn 2 antibody
      • Lcn2 antibody
      • Lipocalin-2 antibody
      • Migration stimulating factor inhibitor antibody
      • MSFI antibody
      • Neutrophil gelatinase associated lipocalin antibody
      • Neutrophil gelatinase associated lipocalin precursor antibody
      • Neutrophil gelatinase-associated lipocalin antibody
      • NGAL antibody
      • NGAL_HUMAN antibody
      • Oncogene 24p3 antibody
      • Oncogenic lipocalin 24p3 antibody
      • p25 antibody
      • Siderocalin antibody
      • siderocalin LCN2 antibody
      • SV40 induced 24P3 protein antibody
      • Uterocalin antibody
      see all

    Anti-Lipocalin-2 / NGAL antibody images

    • ab93963, at 1/100 dilution, staining Lipocalin 2 / NGAL in formalin-fixed, paraffin-embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.

    References for Anti-Lipocalin-2 / NGAL antibody (ab93963)

    ab93963 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab93963.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"