Anti-Lipocalin-2 / NGAL antibody [ABS 039-08] (ab53500)
Key features and details
- Mouse monoclonal [ABS 039-08] to Lipocalin-2 / NGAL
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-Lipocalin-2 / NGAL antibody [ABS 039-08]
See all Lipocalin-2 / NGAL primary antibodies -
Description
Mouse monoclonal [ABS 039-08] to Lipocalin-2 / NGAL -
Host species
Mouse -
Specificity
This antibody reacts with Lipocalin-2 / NGAL -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Does not react with: Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Rat Lipocalin-2/ NGAL (C terminal). His-tag coupled to carrier
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General notes
FUSION PARTNER: Sp2/mIL-6.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABS 039-08 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Database links
- Entrez Gene: 170496 Rat
- SwissProt: P30152 Rat
- Unigene: 11303 Rat
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Alternative names
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab53500 has not yet been referenced specifically in any publications.