Recombinant
RabMAb

Anti-Lipocalin-2 / NGAL antibody [EPR5084] (ab125075)

Overview

  • Product name
    Anti-Lipocalin-2 / NGAL antibody [EPR5084]
    See all Lipocalin-2 / NGAL primary antibodies
  • Description
    Rabbit monoclonal [EPR5084] to Lipocalin-2 / NGAL
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Lipocalin-2/ NGAL aa 50-150.
    (Peptide available as ab187960)

  • Positive control
    • Human fetal kidney lysate and SW480 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Lipocalin-2 / NGAL antibody (Alexa Fluor® 647) [EPR5084] (ab206267)

    Anti-Lipocalin-2 / NGAL antibody (Alexa Fluor® 488) [EPR5084] (ab206085)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab125075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.Can be blocked with Lipocalin-2 / NGAL peptide (ab187960).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
    • Tissue specificity
      Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the calycin superfamily. Lipocalin family.
    • Cellular localization
      Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
    • Information by UniProt
    • Database links
    • Alternative names
      • 24p3 antibody
      • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
      • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
      • Alpha 2 microglobulin related protein antibody
      • HGNC:6526 antibody
      • HNL antibody
      • Lcn 2 antibody
      • Lcn2 antibody
      • Lipocalin-2 antibody
      • Migration stimulating factor inhibitor antibody
      • MSFI antibody
      • Neutrophil gelatinase associated lipocalin antibody
      • Neutrophil gelatinase associated lipocalin precursor antibody
      • Neutrophil gelatinase-associated lipocalin antibody
      • NGAL antibody
      • NGAL_HUMAN antibody
      • Oncogene 24p3 antibody
      • Oncogenic lipocalin 24p3 antibody
      • p25 antibody
      • Siderocalin antibody
      • siderocalin LCN2 antibody
      • SV40 induced 24P3 protein antibody
      • Uterocalin antibody
      see all

    Images

    • All lanes : Anti-Lipocalin-2 / NGAL antibody [EPR5084] (ab125075) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 23 kDa

    References

    This product has been referenced in:
    • Ku AT  et al. TCF7L1 promotes skin tumorigenesis independently of ß-catenin through induction of LCN2. Elife 6:N/A (2017). WB . Read more (PubMed: 28467300) »
    • Wei T  et al. Elevated Expression of Matrix Metalloproteinase-9 not Matrix Metalloproteinase-2 Contributes to Progression of Extracranial Arteriovenous Malformation. Sci Rep 6:24378 (2016). WB . Read more (PubMed: 27075045) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab125075.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up