SpecificityRecognizes native lipoic acid and lipoic acid covalently attached to proteins. It does not recognize lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceLipoic acid is a sulfur-containing substance that is readily converted to and from its reduced form, dihydrolipoic acid. It acts as a coenzyme in reactions that occur in the Krebs cycle; specifically it is involved in the decarboxylation of pyruvate and some other alpha-keto acids. Protein-bound lipoic acids such as E2 subunits, a-ketoglutarate dehydrogenase, or pyruvate dehydrogenase have been shown to be highly susceptible to free radical-derived oxidative modification. Lipoic acid is able to deactivate both fat and water soluble free radicals and thereby protect both lipoproteins and membranes; no other anti-oxidant can do this. It has also shown great promise in the treatment of HIV and in diabetes.
References for Anti-Lipoic Acid antibody (ab58724)
This product has been referenced in:
Lacroix M et al. E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity. Proc Natl Acad Sci U S A113:10998-1003 (2016).
Read more (PubMed: 27621446) »
Ajit Bolar N et al. Mutation of the iron-sulfur cluster assembly gene IBA57 causes severe myopathy and encephalopathy. Hum Mol Genet22:2590-602 (2013).
Read more (PubMed: 23462291) »