Anti-Lipoprotein a antibody (Biotin) (ab27631)

Overview

  • Product nameAnti-Lipoprotein a antibody (Biotin)
    See all Lipoprotein a primary antibodies
  • Description
    Sheep polyclonal to Lipoprotein a (Biotin)
  • ConjugationBiotin
  • SpecificityThsi antibody specifically binds to human Lipoprotein a.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native Lipoprotein a protein (purified) (Human)

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab27631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000.
ELISA 1/5000 - 1/20000.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • RelevanceLipoprotein(a) (Lp(a)) is a lipoprotein subclass assembled in the blood from low density lipoprotein (LDL) molecules and apolipoprotein-a (apo-a). Lp(a) recruits inflammatory cells through interaction with Mac-1 integrin. High Lp(a) in blood is a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis, and stroke. Lp(a) concentrations may be affected by disease states, but are only moderately affected by diet, exercise and other environmental factors. Lipid-reducing drugs have no effect on Lp(a) concentration. High Lp(a) predicts risk of early atherosclerosis similar to high LDL, but in advanced atherosclerosis, Lp(a) is a risk factor independent of LDL, indicating a coagulant risk of plaque thrombosis. Apo(a) contains domains that are very similar to plasminogen (PLG). Lp(a) accumulates in the vessel wall and inhibits binding of PLG to the cell surface, reducing plasmin generation which increases clotting. This inhibition also promotes proliferation of smooth muscle cells. These unique features of Lp(a) suggest a role in the generation of clots and atherosclerosis.
  • Database links
  • Alternative names
    • AK38 antibody
    • Antiangiogenic AK38 protein antibody
    • apo(a) antibody
    • APOA antibody
    • apolipoprotein(a) antibody
    • LP antibody
    • lp(a) antibody
    • LPA antibody
    see all

Anti-Lipoprotein a antibody (Biotin) images

  • ICC/IF image of ab27631 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27631 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- Sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Lipoprotein a antibody (Biotin) (ab27631)

ab27631 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization No
Specification Liver
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization No
Specification Liver
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"