Specificityab78236 reacted with Lipoprotein H2 on 50 of the 52 strains of P. aeruginosa that were tested. The antibody also binds to the P. fluorescens group of the Family Pseudomonadaceae and with Azotobacter vinelandii, but not with other gram negative bacteria.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
RelevancePseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an opportunistic pathogen of plants. P. aeruginosa bacteria are clinically important because they are resistant to most antibiotics and they are capable of surviving in conditions that few other organisms can tolerate. Pseudomonas is often encountered in hospital and clinical work because it is a major cause of hospital acquired (nosocomal) infections. Its main targets are immunocompromised individuals, burn victims, and individuals on respirators or with indwelling catheters. Additionally, these pathogens colonize the lungs of cystic fibrosis patients.
P. aeruginosa is often identified by its pearlescent appearance and grape-like odor in vitro. Definitive clinical identification of P. aeruginosa includes identifying the production of both pyocyanin and fluorescein as well as its ability to grow at 42°C. P. aeruginosa is capable of growth in diesel and jet fuel, where it is known as hydrocarbon utilizing microorganisms (or "HUM bugs"), causing microbial corrosion. It creates dark gellish mats sometimes improperly called "algae".
Lipoprotein H2 is found in the outer membrane.
References for Anti-Lipoprotein H2 antibody [MA1-6] (ab78236)
This product has been referenced in:
Hancock RE et al. Monoclonal antibodies against Pseudomonas aeruginosa outer membrane antigens: isolation and characterization. Infect Immun37:166-71 (1982).
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