Overview

  • Product name
    Anti-Liprin alpha 1 antibody
    See all Liprin alpha 1 primary antibodies
  • Description
    Chicken polyclonal to Liprin alpha 1
  • Specificity
    The antibody has been tested against an e-coli derived fusion protein and has not been tested against endogenous protein.
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
    Predicted to work with: Xenopus laevis
  • Immunogen

    Recombinant fragment: E AMAQKEDM EE RITTLEK RYL AAQREA TSVH DLNDK LENEI ANKD SMHRQT, corresponding to amino acids 310/360 of Human PPFIA1

  • Positive control
    • fusion protein

Properties

Applications

Our Abpromise guarantee covers the use of ab26192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 136 kDa.
ICC 1/200.

Target

  • Function
    May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the liprin family. Liprin-alpha subfamily.
    Contains 3 SAM (sterile alpha motif) domains.
  • Domain
    The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS.
  • Cellular localization
    Cytoplasm. Colocalizes with PTPRF at the ends of focal adhesions most proximal to the cell nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ42630 antibody
    • HGNC:9245 antibody
    • LAR interacting protein 1 antibody
    • LAR-interacting protein 1 antibody
    • LIP 1 antibody
    • LIP-1 antibody
    • LIP.1 antibody
    • LIP1 antibody
    • LIPA1_HUMAN antibody
    • LIPRIN antibody
    • Liprin-alpha-1 antibody
    • MGC26800 antibody
    • PPFIA 1 antibody
    • PPFIA1 antibody
    • Protein tyrosine phosphatase receptor type f polypeptide (PTPRF) interacting protein (liprin) alpha 1 antibody
    • Protein tyrosine phosphatase receptor type f polypeptide interacting protein alpha 1 antibody
    • Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-1 antibody
    • PTPRF interacting protein alpha 1 antibody
    • PTPRF-interacting protein alpha-1 antibody
    see all

References

This product has been referenced in:
  • Sakamoto S  et al. Liprin-a controls stress fiber formation by binding to mDia and regulating its membrane localization. J Cell Sci 125:108-20 (2012). WB ; Human . Read more (PubMed: 22266902) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - other (whole embryo)
Loading amount
15 µg
Specification
whole embryo
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 17 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Embryo (whole tissue)
Specification
whole tissue
Username

Abcam user community

Verified customer

Submitted Jul 16 2012

DISCOUNT CODE: ******
Expiration date: *******

I am very pleased to hear you would like to accept our offer and test ab26192 in Drosophila. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer...

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Thank you very much for your interest in ab26192 and please find the details of the 100% Testing Discount that we talked about on the telephone.

To our knowledge, ab26192 has not been tested in Drosophila. Therefore, I can offer a discount o...

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Thank you for your enquiry. The antibody has not been tested on Drosophila melanogaster and we have not received feedback from customers using this antibody with that species. We do not offer free or trial sized samples for testing purposes. Our po...

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Thank you for your enquiry. The reference on the datasheet for ab26192 is a general reference on liprin alpha 1, it does not refer to the antibody I'm afraid. I'm sorry this caused confusion. We are not aware of publications having used this antibody...

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