Overview

  • Product name
    Anti-LIPT1 antibody
  • Description
    Rabbit polyclonal to LIPT1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: TDGTFLSSLL KSPYQGIRSN ATASIPSLVK NLLEKDPTLT CEVLMNAVAT EYAAYHQIDN HIHLINPTDE TLFPGINSKA KELQTWEWIY GKTPK, corresponding to amino acids 184-278 of Human LIPT1.

  • Positive control
    • Human gall bladder tissue; RT-4 cell lysate
  • General notes


    ab122233 is mono-specific.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    ab122233 is mono-specific.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Catalyzes the transfer of the lipoyl group from lipoyl-AMP to the specific lysine residue of lipoyl domains of lipoate-dependent enzymes.
  • Tissue specificity
    Highly expressed in skeletal muscle and heart, moderately in kidney and pancreas, and detected at lower levels in liver, brain, placenta and lung.
  • Pathway
    Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.
  • Sequence similarities
    Belongs to the lplA family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lipoate biosynthesis protein antibody
    • Lipoate-protein ligase antibody
    • Lipoyl ligase antibody
    • Lipoyltransferase 1 antibody
    • Lipoyltransferase 1 mitochondrial antibody
    • LIPT_HUMAN antibody
    • Lipt1 antibody
    • mitochondrial antibody
    see all

Images

  • ab122233, at 1/50 dilution, staining LIPT1 in paraffin-embedded Human gall bladder tissue by Immunohistochemistry.
  • All lanes : Anti-LIPT1 antibody (ab122233) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

References

ab122233 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab122233.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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