Overview

  • Product nameAnti-Listeria monocytogenes antibody
    See all Listeria monocytogenes primary antibodies
  • Description
    Rabbit polyclonal to Listeria monocytogenes
  • SpecificityThis antibody is specific for Listeria monocytogenes whole cells. This antiserum has not been absorbed and may react with related microorganisms.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Listeria monocytogenes.
  • Immunogen

    Tissue/ cell preparation

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notes>95% pure.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab35132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
ICC/IF
  • Application notesELISA: 1/500 - 1/2000.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution. (Pre-treatment with 0.1% pronase was used)
    WB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
    • Alternative names
      • L. monocytogenes antibody

    References for Anti-Listeria monocytogenes antibody (ab35132)

    This product has been referenced in:
    • Almeida MT  et al. Src-dependent tyrosine phosphorylation of non-muscle myosin heavy chain-IIA restricts Listeria monocytogenes cellular infection. J Biol Chem 290:8383-95 (2015). Read more (PubMed: 25635050) »
    • Köprülü AD  et al. The tyrosine kinase Btk regulates the macrophage response to Listeria monocytogenes infection. PLoS One 8:e60476 (2013). ICC/IF ; Listeria monocytogenes . Read more (PubMed: 23544144) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

    Thank you for your enquiry and your interest in our product. I can confirm that the concentration of this batch is 4.0 mg/ml. I hope this helps and if I can assist further, please do not hesitate to contact me.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"