The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDirect ELISA: Use at a concentration of 1ng/ml - 3 µg/ml.
Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at + 4 °C. Add ab11438 in PBST, 1 h, 37 °C.
Use anti-mouse IgG HRP-conjugated in PBST 1 hr, 37 °C. Substrate: TMB, 30 min.
WB: Use at a concentration of 8 µg/ml. Use 15 % SDS-PAGE electrophoresis in reducing conditions (30 ?g antigen per track) and transfer to nitrocellulose membrane (60 min, 100 mA). Blocking: 1 % BSA in PBS, 1 h, 37 °C.
Detects a band of approximately 23 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.