Anti-Listeria monocytogenes antibody [LZA2] (ab11438)


  • Product nameAnti-Listeria monocytogenes antibody [LZA2]
    See all Listeria monocytogenes primary antibodies
  • Description
    Mouse monoclonal [LZA2] to Listeria monocytogenes
  • SpecificityRecognises L. Monocytogenes outer membrane fraction and whole cells.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    This antibody does not react with the following species: E. coli, Salmonella, Y. pestis, F. tularensis, L. pneumophila and B. abortus.
  • Immunogen

    Listeria monocytogenes.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityPurified IgM
  • Purification notesClone LZA2 is purified by euglobulin precipitation. Purity tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone numberLZA2
  • MyelomaSp2/0
  • IsotypeIgM
  • Research areas


Our Abpromise guarantee covers the use of ab11438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesDirect ELISA: Use at a concentration of 1ng/ml - 3 µg/ml.
    Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at + 4 °C. Add ab11438 in PBST, 1 h, 37 °C.
    Use anti-mouse IgG HRP-conjugated in PBST 1 hr, 37 °C. Substrate: TMB, 30 min.

    WB: Use at a concentration of 8 µg/ml. Use 15 % SDS-PAGE electrophoresis in reducing conditions (30 ?g antigen per track) and transfer to nitrocellulose membrane (60 min, 100 mA). Blocking: 1 % BSA in PBS, 1 h, 37 °C.
    Detects a band of approximately 23 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
    • Alternative names
      • L. monocytogenes antibody

    References for Anti-Listeria monocytogenes antibody [LZA2] (ab11438)

    ab11438 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

    Thank you for your enquiry. The concentration of this lot of the antibody is 4.40 mg/ml. The size we sell is 100ug, but it appears that you actually received nearly double that amount! I hope this information helps. Please do not hesitate to c...

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    I enclose the protocols that were used to test these antibodies: Direct ELISA: Use at a concentration of 1ng/ml - 3 µg/ml. Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at +4°C. Add ab...

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