Anti-Listeria monocytogenes antibody [LZF7] (HRP) (ab24456)

Overview

  • Product name
    Anti-Listeria monocytogenes antibody [LZF7] (HRP)
    See all Listeria monocytogenes primary antibodies
  • Description
    Mouse monoclonal [LZF7] to Listeria monocytogenes (HRP)
  • Conjugation
    HRP
  • Specificity
    Reacts with L. Monocytogenes outer membrane fraction and intact cells.
  • Tested applications
    Suitable for: ELISA, WB, Sandwich ELISAmore details
  • Species reactivity
  • Immunogen

    Listeria monocytogenes.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, PBS, 10mg/ml BSA, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    LZF7
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
Sandwich ELISA Use at an assay dependent dilution. Can be used as Detection antibody with recommended pair.

Target

  • Relevance
    The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
  • Alternative names
    • L. monocytogenes antibody

References

ab24456 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Antibody ab11439 (LZH1 clone) was used for capture and ab24456 for detection. The antibodies were tested at various dilutions.

ab11439: Use a concentration of 0.001 - 3 µg/ml.

ab24456: Use a concentration of 1ng/ml - 3 µg/ml.
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We have a report that these antibodies are working in ELISA and WB at least with heat-killed Listeria monocytogenes cells from KPL Inc. (Cat.# 50-90-90).

Thank you for contacting us with your question, and for your patience while I have been in touch with the lab regarding this antibody. This antibody tested negative by ELISA for cross-reactivity to: E. coli, Salmonella, Y. pestis, F. tularensis, L. pne...

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Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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