Anti-Listeria monocytogenes antibody [LZH1] (ab11439)


  • Product name
    Anti-Listeria monocytogenes antibody [LZH1]
    See all Listeria monocytogenes primary antibodies
  • Description
    Mouse monoclonal [LZH1] to Listeria monocytogenes
  • Specificity
    Reacts with L. Monocytogenes outer membrane fraction and intact cells.
  • Tested applications
    Suitable for: ELISA, WB, Sandwich ELISAmore details
  • Species reactivity
    This antibody does not react with the following species: E. coli, Salmonella, Y. pestis, F. tularensis, L. pneumophila and B. abortus. Ab11439 does react with L. innocua and L. ivanovii.
  • Immunogen

    Listeria monocytogenes.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purity tested by electrophoresis.
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab11439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.001 - 3 µg/ml. Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at + 4 °C. Add Mouse monoclonal [LZA2] to Listeria monocytogenes in PBST, 1 h, 37 °C.
WB Use a concentration of 8 µg/ml. Predicted molecular weight: 23 kDa. Use 15 % SDS-PAGE electrophoresis in reducing conditions (30 ug antigen per track) and transfer to nitrocellulose membrane (60 min, 100 mA). Blocking: 1 % BSA in PBS, 1 h, 37 °C.
Sandwich ELISA Use a concentration of 0.001 - 3 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [LZF7] to Listeria monocytogenes (HRP) (ab24456). Use this Capture antibody at a concentration of 1ng/ml - 3 µg/ml.


  • Relevance
    The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
  • Alternative names
    • L. monocytogenes antibody

References for Anti-Listeria monocytogenes antibody [LZH1] (ab11439)

ab11439 has not yet been referenced specifically in any publications.

Product Wall

Antibody ab11439 (LZH1 clone) was used for capture and ab24456 for detection. The antibodies were tested at various dilutions.

ab11439: Use a concentration of 0.001 - 3 µg/ml.

ab24456: Use a concentration of 1ng/ml - 3 µg/ml.
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We have a report that these antibodies are working in ELISA and WB at least with heat-killed Listeria monocytogenes cells from KPL Inc. (Cat.# 50-90-90).

Thank you for contacting us.

I have been informed by the source of the antibody ab11439 that the outer membrane fraction mentioned on the datasheet is a complex antigen of the Listeria monocytogenes cell membrane. Sorry about the confusing nam...

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Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Listeria monocytogenes Cell (Bacteria)
Yes - Triton-X 0.5%

Abcam user community

Verified customer

Submitted Aug 31 2011

Thank you for your enquiry, we do not supply a positive control with this Ab; however I am in contact with the originator in order to confirm what was used as a positive control in ELISA during testing. I will be in touch again when I have a response. ...

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I enclose the protocols that were used to test these antibodies: Direct ELISA: Use at a concentration of 1ng/ml - 3 µg/ml. Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at +4°C. Add ab...

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Thank you for your enquiry regarding our Salmonella typhimurium, Listeria monocytogenes, or Campylobacter jejuni antibodies. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (ab11440)(capture – conjugate) for sandwich imm...

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