Our Abpromise guarantee covers the use of ab11439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.001 - 3 µg/ml. Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at + 4 °C. Add Mouse monoclonal [LZA2] to Listeria monocytogenes in PBST, 1 h, 37 °C.|
|WB||Use a concentration of 8 µg/ml. Predicted molecular weight: 23 kDa. Use 15 % SDS-PAGE electrophoresis in reducing conditions (30 ug antigen per track) and transfer to nitrocellulose membrane (60 min, 100 mA). Blocking: 1 % BSA in PBS, 1 h, 37 °C.|
|Sandwich ELISA||Use a concentration of 0.001 - 3 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [LZF7] to Listeria monocytogenes (HRP) (ab24456). Use this Capture antibody at a concentration of 1ng/ml - 3 µg/ml.|
ab11439 has not yet been referenced specifically in any publications.