Live Cell Labeling Kit - Blue Fluorescence - Cytopainter (ab187963)

Overview

  • Product name
    Live Cell Labeling Kit - Blue Fluorescence - Cytopainter
    See all Cell labeling kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Assay time
    2h 00m
  • Assay duration
    Multiple steps standard assay
  • Product overview

    Live Cell Labeling Kit - Blue Fluorescence | Cytopainter (ab187963) is designed to uniformly label live cells in blue fluorescence with a proprietary dye whose fluorescence is strongly enhanced upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. Cells grown on black wall/clear bottom plates or slides can be stained and quantified in less than two hours. View cells at Ex/Em = 360/445 nm.


    This kit can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both suspension and adherent cells.

  • Notes

    Abcam’s CytoPainter Live Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations and flow cytometric investigations of cellular functions. The effective labelling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab187963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Image of CPA cells stained with CytoPainter Live Cell Labeling Kit - Blue Fluorescence (ab187963) in a black wall/clear bottom 96-well plate.

Protocols

References

ab187963 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187963.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up