Live Cell Labeling Kit - Orange Fluorescence (Ex405nm) - Cytopainter (ab187968)

Overview

  • Product name
    Live Cell Labeling Kit - Orange Fluorescence (Ex405nm) - Cytopainter
    See all Cell labeling kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Product overview

    Live Cell Labeling Kit - Orange Fluorescence (Ex405nm) | Cytopainter (ab187698) is designed to uniformly label live cells in orange fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time. The kit uses a non-fluorescent dye that carries a cell-retaining moiety. The dye becomes strongly fluorescent upon entering into live cells, and is trapped inside cells to give stable fluorescence signals. The dye is a hydrophobic compound that easily permeates intact live cells. The labeling process is robust, requiring minimal hands-on time. The orange fluorescence dye has the spectral properties of large Stokes Shift at Ex/Em = ~400/550 nm. When well excited with the Violet Laser at 405 nm, the dye emits intense orange fluorescence at ~550 nm. The kit is optimized to be used with a flow cytometer equipped with a Violet Laser and particularly suitable for multicolor flow cytometric analysis of cells. It can also be used with a fluorescence microscope with a customized filter set.


    The kit provides all the essential components with an optimized cell-labeling protocol (Ex/Em= 405/550 nm). It is an excellent tool to label cells for fluorescence microscopic and flow cytometric investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. The kit is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells.

  • Notes

    Abcam’s CytoPainter Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab187968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Images

Protocols

References

ab187968 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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