Live/Dead Cell Assay (ab115347)
- Product nameLive/Dead Cell AssaySee all Live dead cell kits ...
- Detection methodFluorescent
- Product overview
ab115347 is a one-step assay to differentially labels live and dead cells with fluorescent dyes. It is useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy. The provided Live/Dead Assay stain is sufficient for ~1000 assays.
The Live/Dead assay stain solution is a mixture of two highly fluorescent dyes that differentially label live and dead cells:
The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm, respectively (similar to FITC).
The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm, respectively.
The Live/Dead Cell Assay is a one-step staining procedure that is simple and fast. It can be used directly in cell culture media. Stained cells are not compatible with fixation.
Store the Live/Dead staining solution at 4°C in the dark. The
product is stable for 6 months from receipt. For longer term storage,
aliquot and store at -20°C or -80°C. Avoid multiple freeze-thaw
cycles. Allow the product to warm to room temperature before
opening. Use diluted solutions of Live/Dead stain promptly.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1000 tests 1000X Live/Dead Cell stain in DMSO 1 x 0.1ml
- Research Areas
- RelevanceDistinguishing between live and dead cells is very important for investigation of growth control and cell death.
Our Abpromise guarantee covers the use of ab115347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Live/Dead Cell Assay images
Jurkat cells were treated with a dose response of Etoposide on day 0 and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry. At each time point a small aliquot of cells was removed from the culture for analysis.
(A) Dot plots showing live/dead analysis of day 3 samples. Live cells are on the y-axis and dead cells are on the x-axis. The red polygongate identifies live cells and the number indicates the percent of live cells.
Flow cytometry analysis using the Live/Dead assay stain. Jurkat cells were treated with a dose response of Etoposide on day 0 and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry. At each time point a small aliquot of cells was removed from the culture for analysis.
Graphical representation of the data presented in (A) with additional time points. % viable cells are plotted relative to time, with respect to Etoposide concentration.
The sample dot plots demonstrate varying ratios of live and dead cells.
Jurkat cells stained with the live/dead assay kit. Jurkat cells treated with Etoposide were labeled with the live/dead assay stain. Live cells (with esterase activity) stain green and dead cells (compromised plasma membrane) stain red. (A) Field of cells following 10 minute staining in media of live/dead stain. (B)Magnified view showing that in live cells the whole cell is stained green whereas in dead red cells it is the fragmented nuclear DNA that is stained.
References for Live/Dead Cell Assay (ab115347)
ab115347 has not yet been referenced specifically in any publications.