Overview

  • Product nameLive/Dead Cell AssaySee all Live dead cell kits ...
  • Detection methodFluorescent
  • Product overview

    ab115347 is a one-step assay to differentially labels live and dead cells with fluorescent dyes. It is useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy. The provided Live/Dead Assay stain is sufficient for ~1000 assays.

    The Live/Dead assay stain solution is a mixture of two highly fluorescent dyes that differentially label live and dead cells:

    The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm, respectively (similar to FITC).

    The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm, respectively.

    The Live/Dead Cell Assay is a one-step staining procedure that is simple and fast. It can be used directly in cell culture media. Stained cells are not compatible with fixation.

  • Notes

    Store the Live/Dead staining solution at 4°C in the dark. The

    product is stable for 6 months from receipt. For longer term storage,

    aliquot and store at -20°C or -80°C. Avoid multiple freeze-thaw

    cycles. Allow the product to warm to room temperature before

    opening. Use diluted solutions of Live/Dead stain promptly.

  • Tested applicationsFunctional Studiesmore details
  • PlatformReagents

Properties

  • RelevanceDistinguishing between live and dead cells is very important for investigation of growth control and cell death.
  • Alternative names
    • MS961

Applications

Our Abpromise guarantee covers the use of ab115347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Live/Dead Cell Assay images

  • Jurkat cells were treated with a dose response of Etoposide on day 0 and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry. At each time point a small aliquot of cells was removed from the culture for analysis.
    (A) Dot plots showing live/dead analysis of day 3 samples. Live cells are on the y-axis and dead cells are on the x-axis. The red polygongate identifies live cells and the number indicates the percent of live cells.
  • Flow cytometry analysis using the Live/Dead assay stain. Jurkat cells were treated with a dose response of Etoposide on day 0 and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry. At each time point a small aliquot of cells was removed from the culture for analysis.
    Graphical representation of the data presented in (A) with additional time points. % viable cells are plotted relative to time, with respect to Etoposide concentration.
  • The sample dot plots demonstrate varying ratios of live and dead cells.
  • Jurkat cells stained with the live/dead assay kit. Jurkat cells treated with Etoposide were labeled with the live/dead assay stain. Live cells (with esterase activity) stain green and dead cells (compromised plasma membrane) stain red. (A) Field of cells following 10 minute staining in media of live/dead stain. (B)Magnified view showing that in live cells the whole cell is stained green whereas in dead red cells it is the fragmented nuclear DNA that is stained.

Protocols

References for Live/Dead Cell Assay (ab115347)

This product has been referenced in:
  • Singh D  et al. Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing. Evid Based Complement Alternat Med 2014:701656 (2014). Human . Read more (PubMed: 24719644) »
  • Sun W  et al. Sox9 gene transfer enhanced regenerative effect of bone marrow mesenchymal stem cells on the degenerated intervertebral disc in a rabbit model. PLoS One 9:e93570 (2014). Read more (PubMed: 24691466) »

See all 3 Publications for this product

Product Wall

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You can use same FITC and PE filters in this assay.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Unfortunately, we do not have free or trial sized samples of any of our products. We will guarantee this kit for differentiating between live and dead cells. If you are at all unsatisfied with the results, we are ...

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I have sent refund request to our accounts department on 13th June 2012. If you haven't received the refund please contact them by email mailto:creditcontrol@abcam.com or call 01223-696900 for accounts.

I ...

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Your credit note ID is XXXXXXX

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used ...

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Thank you for contacting us. I have been discussing this case with my colleagues, who developed this product; the following is their reply

-try staining the cells in PBS+dye (aspirate media, replace with 5X dye in PBS) [2b in microscopy secti...

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Thank you for your email. I am sorry to hear that you are experiencing problems with this kit.

I have checked the protocol and details you have kindly provided. It seems the dye is not entering the cells, which could be due to cells so is it ...

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Thank you for your enquiry. I am sorry to confirm we have not tested cell viability post-staining with this product and we would not be able to guarantee whether or notit would affect the cells you want to continue culturing. Ifyou would liketo test it...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"