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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
ab115347 is a one-step assay to differentially labels live and dead cells with fluorescent dyes. It is useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy. The provided Live/Dead Assay stain is sufficient for ~1000 assays.
The Live/Dead assay stain solution is a mixture of two highly fluorescent dyes that differentially label live and dead cells:
The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm, respectively (similar to FITC).
The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm, respectively.
The Live/Dead Cell Assay is a one-step staining procedure that is simple and fast. It can be used directly in cell culture media. Stained cells are not compatible with fixation.
Store the Live/Dead staining solution at 4°C in the dark. The
product is stable for 6 months from receipt. For longer term storage,
aliquot and store at -20°C or -80°C. Avoid multiple freeze-thaw
cycles. Allow the product to warm to room temperature before
opening. Use diluted solutions of Live/Dead stain promptly.
|1000X Live/Dead Cell stain in DMSO||1 x 0.1ml|
Our Abpromise guarantee covers the use of ab115347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"