Recombinant Anti-liver FABP antibody [EPR5895(2)] (ab129203)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5895(2)] to liver FABP
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-liver FABP antibody [EPR5895(2)]
See all liver FABP primary antibodies -
Description
Rabbit monoclonal [EPR5895(2)] to liver FABP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human liver FABP aa 50-150. The exact sequence is proprietary.
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Positive control
- Fetal liver, Mouse liver, Human small intestine and HepG2 whole cell lysate (ab7900); Permeabilized HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.55 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5895(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
Target
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Function
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2168 Human
- Entrez Gene: 14080 Mouse
- Omim: 134650 Human
- SwissProt: P07148 Human
- SwissProt: P12710 Mouse
- Unigene: 380135 Human
- Unigene: 22126 Mouse
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Alternative names
- FABP 1 antibody
- FABP1 antibody
- FABPL antibody
see all
Images
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All lanes : Anti-liver FABP antibody [EPR5895(2)] (ab129203) at 1/1000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Human small intestine lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa -
ab129203, at 1/10 dilution, staining FABP in permeabilized HepG2 cells by intracellular flow cytometry (Red). Green represents rabbit IgG negative control.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab129203 has been referenced in 3 publications.
- Murphy S et al. Proteomic profiling of liver tissue from the mdx-4cv mouse model of Duchenne muscular dystrophy. Clin Proteomics 15:34 (2018). PubMed: 30386187
- Beyaz S et al. High-fat diet enhances stemness and tumorigenicity of intestinal progenitors. Nature 531:53-8 (2016). PubMed: 26935695
- Wang YG & Yang TL Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway. Exp Ther Med 10:1777-1783 (2015). WB ; Human . PubMed: 26640549