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ab7847 has been referenced in 3 publications.
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ICC/IF image of ab7847 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7847, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-liver FABP antibody (ab7847) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from pig liver
Lane 2 : Whole tissue lysate prepared from pig liver
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Image courtesy of an anonymous Abreview.
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