Anti-liver FABP antibody (ab7847)

Can you tell me if full length rat liver FABP was used as an immunogen?

ANSWER:

Thank you for your enquiry.

I can confirm that natural rat L-FABP was used. L-FABP was isolated and purified from livers of Wistar strain rats as described in: Takashi, K. et al; Isolation and characterization of the three fractions (DE-I, DE-II and DE-III) of rat liver Z-protein and the complete primary structure of DE-II. Eur. J. Biochem. 1983; 136, 589-601.

As I have limited access to journals, I hope the publication above will provide you with the information you are looking for.

We need to know either the immunogen or peptide sequence used to make this antibody

ANSWER:

Thank you for your enquiry regarding ab7847. To answer your question, polyclonal antibody to rat liver FABP natural rat L-FABP was used. L-FABP was isolated and purified from livers of Wistar strain rats as described in: Takahashi K et al; Isolation and characterization of the three fractions (DE-I, DE-II and DE-III) of rat liver Z-protein and the complete primary structure of DE-II., Eur. J. Biochem. 1983; 136. pag 589-601.

We hope this information will be helpful to you. Please contact us again for further assistance.

Customer would like to know how how this antibody was purified.

ANSWER:

Ab7847 was Protein A purified. If you have any more questions, please contact us again.

I'm trying to find some antibodies (monoclonal or polyclonal) which are specific enough to distinguish the various members of Fatty Acid Binding Proteins (FABP). I find some in your website, such as ab7847 and ab7366. However, I can't get enough information about their specificity. In my knowledge, there are at least eight members of FABP family in human, they are heart-FABP, liver-FABP,intestinal-FABP,adipocyte-FABP,psoriasis-FABP,gastrotropin, PMP2 and brain-FABP. In your data sheet, you descibed the antobodies as "no cross-reactivities with other FABP types have been detected". I want to know if that indicated that you have ckecked all the eight FABP members for the specificity. I'm looking forward to hearing from you. Thanks.

ANSWER:

Both of these antibodies to L-FABP (ab7847 and ab7366) have been tested for cross reactivity with H-FABP, I-FABP. L-FABP and I-FABP are the most similar FABP's of the eight members of the FABP family. So it is very unlikely that the antibodies cross react with other members of the FABP family. However, cross reactivity with other FABP proteins has not specifically been tested - I shall make this more clear on the datasheet for ab7847.