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Products:Signal Transduction >> Metabolism >> Lipid metabolism
Overview
Product name
Anti-liver FABP antibody
See all liver FABP products (8) ...
Description
Rabbit polyclonal to liver FABP
Specificity
The antibody reacts with rat L-FABP of both natural and recombinant origin. Localization of FABP is demonstrated in mucosa of small and large intestine and in intestinal metaplasia of the gastric mucosa. Tested for cross reactivity with H-FABP and I-FABP. L-FABP and I-FABP are the most similar FABP's of the eight members of the FABP family. So it is very unlikely that the antibodies cross react with other members of the FABP family.
Tested applications
ICC/IF, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.2% BSA, 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Target
Function
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP07148
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FABP1 antibody
- liver antibody
- FABP 1 antibody
see all
Database links
- Entrez Gene: 2168 Human
- Entrez Gene: 14080 Mouse
- Entrez Gene: 24360 Rat
- Omim: 134650 Human
- SwissProt: P07148 Human
- SwissProt: P12710 Mouse
- SwissProt: P02692 Rat
- Unigene: 380135 Human
see all
Anti-liver FABP antibody images:
Immunocytochemistry/ Immunofluorescence - liver FABP antibody (ab7847)
ICC/IF image of ab7847 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7847, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - liver FABP antibody (ab7847)
All lanes : Anti-liver FABP antibody (ab7847) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from pig liver
Lane 2 : Whole tissue lysate prepared from pig liver
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Image courtesy of an anonymous Abreview.
References for Anti-liver FABP antibody (ab7847)
This product has been referenced in:
- Chattopadhyay Met al. Ablation of PI3K p110-a prevents high-fat diet-induced liver steatosis. Diabetes 60:1483-92 (2011).Read more (PubMed: 21464441) »
- Perra Aet al. Thyroid hormone (T3) and TRbeta agonist GC-1 inhibit/reverse nonalcoholic fatty liver in rats. FASEB J 22:2981-9 (2008). WB; Rat.Read more (PubMed: 18434432) »
See all 3 publications for this product
Publishing research using ab7847? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"