Anti-Liver Arginase antibody (ab92274)

Overview

  • Product name
    Anti-Liver Arginase antibody
    See all Liver Arginase primary antibodies
  • Description
    Goat polyclonal to Liver Arginase
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide:

    CFGLAREGNHKPID

    , corresponding to C terminal amino acids 303-316 of Human liver Arginase (NP_000036.2).

  • Positive control
    • Human liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab92274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.

Target

  • Pathway
    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in disease
    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • Sequence similarities
    Belongs to the arginase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

Anti-Liver Arginase antibody images

  • Immunohistochemistry (paraffin-embedded sections) analysis of human kidney tissue labeling Liver Arginase with ab92274 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH9. HRP-staining.

  • All lanes : Anti-Liver Arginase antibody (ab92274) at 0.03 µg/ml

    Lane 1 : pig liver tissue lysate
    Lane 2 : mouse liver tissue lysate
    Lane 3 : rat liver tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Anti-Liver Arginase antibody (ab92274) at 0.01 µg/ml + human liver lysate in RIPA buffer at 35 µg

    Predicted band size : 35 kDa

References for Anti-Liver Arginase antibody (ab92274)

ab92274 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Pig Tissue lysate - whole (myocardium)
Loading amount
50 µg
Specification
myocardium
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Rahul Dalal

Verified customer

Submitted Sep 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up