The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
Use a concentration of 2 - 4 µg/ml.
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
Immunohistochemistry (paraffin-embedded sections) analysis of human kidney tissue labeling Liver Arginase with ab92274 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH9. HRP-staining.
Western blot - Anti-Liver Arginase antibody (ab92274)
All lanes : Anti-Liver Arginase antibody (ab92274) at 0.03 µg/ml
Lane 1 : pig liver tissue lysate Lane 2 : mouse liver tissue lysate Lane 3 : rat liver tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size : 35 kDa Observed band size : 35 kDa
Western blot - liver Arginase antibody (ab92274)
Anti-Liver Arginase antibody (ab92274) at 0.01 µg/ml + human liver lysate in RIPA buffer at 35 µg