Anti-Liver Arginase antibody (ab96183)
Key features and details
- Rabbit polyclonal to Liver Arginase
- Suitable for: WB, IHC-P, ICC/IF, ELISA, IHC-Fr, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Liver Arginase antibody
See all Liver Arginase primary antibodies -
Description
Rabbit polyclonal to Liver Arginase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, ELISA, IHC-Fr, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human Liver Arginase aa 1-292.
Database link: P05089 -
Positive control
- WB: Raji whole cell lysate. Rat liver tissue extract. Mouse liver extract. IHC-P: Cal27 xenograft. Mouse liver tissue. ICC/IF: HeLa cells. IHC-Fr: Mouse hippocampus tissue. ELISA: HEK-293T whole cell extracts. Flow Cyt: HepG2 cell. IP: HepG2 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
|
|
IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective. |
ICC/IF |
1/100 - 1/1000.
|
|
ELISA |
1/1000 - 1/10000.
|
|
IHC-Fr |
1/100 - 1/1000.
|
|
IP |
1/100 - 1/1000.
|
|
Flow Cyt |
1/50 - 1/200.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective. |
ICC/IF
1/100 - 1/1000. |
ELISA
1/1000 - 1/10000. |
IHC-Fr
1/100 - 1/1000. |
IP
1/100 - 1/1000. |
Flow Cyt
1/50 - 1/200. |
Target
-
Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 383 Human
- Entrez Gene: 11846 Mouse
- Entrez Gene: 29221 Rat
- Omim: 608313 Human
- SwissProt: P05089 Human
- SwissProt: Q61176 Mouse
- SwissProt: P07824 Rat
- Unigene: 440934 Human
see all -
Alternative names
- A I antibody
- Al antibody
- ARG 1 antibody
see all
Images
-
Frozen sections of mouse hippocampus tissue stained for Arginase 1 (green) using ab96183 at 1/250 dilution in immunohistochemical analysis. Counterstained with Fluoroshield with DAPI (blue).
-
Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution + Mouse liver extract at 50 µg
Predicted band size: 35 kDaSeparated by 12% SDS-PAGE.
-
Paraffin embedded mouse liver tissue stained for Arginase 1 using ab96183 at 1/500 dilution in immunohistochemical analysis.
-
Arginase 1 was immunoprecipitated from 5µ of HepG2 whole cell lysate with ab96183. Western blot was performed from the immunoprecipitate using ab96183 at 1/600 dilution. EasyBlot anti-Rabbit IgG, was used as secondary antibody.
-
Flow cytometric analysis of HepG2 cell line labeling ARG1 with ab96183 at 1/50 dilution (red) compared with Isotype Control (black) and an unlabelled control. Dylight 488-conjugated was used as secondary antibody.
-
ELISA analysis of HEK-293T whole cell extracts using ab96183 as a detection antibody with concentration of 1 μg/mL.
-
Anti-Liver Arginase antibody (ab96183) at 1/10000 dilution + Rat liver tissue extract at 50 µg
Predicted band size: 35 kDa -
ab96183, at 1/500 dilution, staining liver Arginase in paraffin-embedded Cal27 xenograft by Immunohistochemistry.
-
Anti-Liver Arginase antibody (ab96183) at 1/3000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS-PAGE -
ab96183, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab96183 has been referenced in 7 publications.
- Tian Y et al. Activation of RARα Receptor Attenuates Neuroinflammation After SAH via Promoting M1-to-M2 Phenotypic Polarization of Microglia and Regulating Mafb/Msr1/PI3K-Akt/NF-κB Pathway. Front Immunol 13:839796 (2022). PubMed: 35237277
- Jiao D et al. Biocompatible reduced graphene oxide stimulated BMSCs induce acceleration of bone remodeling and orthodontic tooth movement through promotion on osteoclastogenesis and angiogenesis. Bioact Mater 15:409-425 (2022). PubMed: 35386350
- Tan Y et al. RNA interference-mediated silencing of DNA methyltransferase 1 attenuates neuropathic pain by accelerating microglia M2 polarization. BMC Neurol 22:376 (2022). PubMed: 36183073
- Ban D et al. Cerium Oxide Nanoparticles Alleviate Neuropathic Pain by Modulating Macrophage Polarization in a Rat SCI Model. J Pain Res 15:3369-3380 (2022). PubMed: 36317164
- Fang W et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8+ T cell exclusion. J Exp Clin Cancer Res 40:4 (2021). PubMed: 33390170
- Zhang W et al. Dihydromyricetin attenuates neuropathic pain via enhancing the transition from M1 to M2 phenotype polarization by potentially elevating ALDH2 activity in vitro and vivo. Ann Transl Med 8:1151 (2020). PubMed: 33241000
- Ma Z et al. Overexpression of Arginase-1 is an indicator of poor prognosis in patients with colorectal cancer. Pathol Res Pract N/A:152383 (2019). PubMed: 30890279