Anti-liver Arginase antibody (ab96183)

Overview

  • Product nameAnti-liver Arginase antibody
    See all Liver Arginase primary antibodies
  • Description
    Rabbit polyclonal to liver Arginase
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1-292 of Human liver Arginase (NP_000036).

  • Positive control
    • Raji whole cell lysate; HeLa cells; Cal27 xenograft; 293T, A431, H1299, HepG2 and Molt-4 cell lines

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective.
ICC/IF 1/100 - 1/200.

Target

  • PathwayNitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in diseaseDefects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • Sequence similaritiesBelongs to the arginase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

Anti-liver Arginase antibody images

  • Anti-liver Arginase antibody (ab96183) at 1/3000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 35 kDa
  • ab96183, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
  • ab96183, at 1/500 dilution, staining liver Arginase in paraffin-embedded Cal27 xenograft by Immunohistochemistry.

References for Anti-liver Arginase antibody (ab96183)

ab96183 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (liver)
Specification liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted May 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"