1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For antigen retrieval, either 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH8.0) will be effective.
1/100 - 1/200.
PathwayNitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Involvement in diseaseDefects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
Sequence similaritiesBelongs to the arginase family.