Anti-Liver Arginase antibody (Biotin) (ab34666)

Overview

  • Product name
    Anti-Liver Arginase antibody (Biotin)
    See all Liver Arginase primary antibodies
  • Description
    Rabbit polyclonal to Liver Arginase (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, Dot blot, ELISA, Immunomicroscopymore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Tissue/ cell preparation liver Arginase (Bovine liver).

  • Positive control
    • Purified and partially purified Arginase [Bovine Liver]
  • General notes

    10-20 Biotin molecules per rabbit IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% (w/v) Sodium Azide
    Constituents: 10mg/ml BSA (IgG and Protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
ELISA
Immunomicroscopy
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/4000.

    This antibody has been assayed against 1.0µg of liver Arginase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.

    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
    Suitable for other antibody based assays using streptavidin or avidin conjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Pathway
      Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
    • Involvement in disease
      Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
    • Sequence similarities
      Belongs to the arginase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Alternative names
      • A I antibody
      • Al antibody
      • ARG 1 antibody
      • arg1 antibody
      • ARGI1_HUMAN antibody
      • Arginase 1 antibody
      • Arginase liver antibody
      • Arginase type I antibody
      • Arginase, liver antibody
      • Arginase-1 antibody
      • Arginase1 antibody
      • Liver type arginase antibody
      • Liver-type arginase antibody
      • Type I arginase antibody
      see all

    References

    ab34666 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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