Anti-Liver Arginase antibody (Biotin) (ab34666)

Overview

  • Product nameAnti-Liver Arginase antibody (Biotin)
    See all Liver Arginase primary antibodies
  • Description
    Rabbit polyclonal to Liver Arginase (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, Dot blot, ELISA, Immunomicroscopymore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Tissue/ cell preparation liver Arginase (Bovine liver).

  • Positive control
    • Purified and partially purified Arginase [Bovine Liver]
  • General notes

    10-20 Biotin molecules per rabbit IgG molecule.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% (w/v) Sodium Azide
    Constituents: 10mg/ml BSA (IgG and Protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
ELISA
Immunomicroscopy
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/4000.

    This antibody has been assayed against 1.0µg of liver Arginase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.

    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
    Suitable for other antibody based assays using streptavidin or avidin conjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • PathwayNitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
    • Involvement in diseaseDefects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
    • Sequence similaritiesBelongs to the arginase family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Alternative names
      • A I antibody
      • Al antibody
      • ARG 1 antibody
      • arg1 antibody
      • ARGI1_HUMAN antibody
      • Arginase 1 antibody
      • Arginase liver antibody
      • Arginase type I antibody
      • Arginase, liver antibody
      • Arginase-1 antibody
      • Arginase1 antibody
      • Liver type arginase antibody
      • Liver-type arginase antibody
      • Type I arginase antibody
      see all

    References for Anti-Liver Arginase antibody (Biotin) (ab34666)

    ab34666 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"