Recombinant
RabMAb

Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)

Overview

  • Product name
    Anti-Liver Arginase antibody [EPR6671(B)]
    See all Liver Arginase primary antibodies
  • Description
    Rabbit monoclonal [EPR6671(B)] to Liver Arginase
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human liver Arginase

  • Positive control
    • Fetal liver and mouse liver lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Pathway
      Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
    • Involvement in disease
      Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
    • Sequence similarities
      Belongs to the arginase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • A I antibody
      • Al antibody
      • ARG 1 antibody
      • arg1 antibody
      • ARGI1_HUMAN antibody
      • Arginase 1 antibody
      • Arginase liver antibody
      • Arginase type I antibody
      • Arginase, liver antibody
      • Arginase-1 antibody
      • Arginase1 antibody
      • Liver type arginase antibody
      • Liver-type arginase antibody
      • Type I arginase antibody
      see all

    Anti-Liver Arginase antibody [EPR6671(B)] images

    • All lanes : Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa

    References for Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)

    This product has been referenced in:
    • Ando Y  et al. Platelet Supernatant Suppresses LPS-Induced Nitric Oxide Production from Macrophages Accompanied by Inhibition of NF-?B Signaling and Increased Arginase-1 Expression. PLoS One 11:e0162208 (2016). Read more (PubMed: 27588757) »
    • Xia N  et al. Uncoupling of Endothelial Nitric Oxide Synthase in Perivascular Adipose Tissue of Diet-Induced Obese Mice. Arterioscler Thromb Vasc Biol 36:78-85 (2016). Read more (PubMed: 26586660) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Bone Marrow-Derived Macrophages)
    Gel Running Conditions
    Reduced Denaturing (10% polyacrylamide gel)
    Loading amount
    160000 cells
    Treatment
    20 ng/mL mouse IL-4 for 24 hours
    Specification
    Bone Marrow-Derived Macrophages
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    祐介 安藤

    Verified customer

    Submitted Nov 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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