Recombinant Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6671(B)] to Liver Arginase
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Liver Arginase antibody [EPR6671(B)]
See all Liver Arginase primary antibodies -
Description
Rabbit monoclonal [EPR6671(B)] to Liver Arginase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human Liver Arginase aa 1-100. The exact sequence is proprietary.
Database link: P05089 -
Positive control
- WB: Human fetal liver and mouse liver lysates and Myc-DDK tagged Recombinant Human ARG1 protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6671(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
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Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 383 Human
- Entrez Gene: 11846 Mouse
- Entrez Gene: 29221 Rat
- Omim: 608313 Human
- SwissProt: P05089 Human
- SwissProt: Q61176 Mouse
- SwissProt: P07824 Rat
- Unigene: 440934 Human
see all -
Alternative names
- A I antibody
- Al antibody
- ARG 1 antibody
see all
Images
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All lanes : Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution
Lane 1 : Wild-type HepG2 cell lysate at 20 µg
Lane 2 : arg1 knockout HepG2 cell lysate at 20 µg
Lane 4 : Human Liver cell lysate at 5 µg
Lane 6 : A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Liver Arginase antibody [EPR6671(B)] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124917 was shown to bind specifically to Liver Arginase. A band was observed at 36 kDa in wild-type HepG2 cell lysates with no signal observed at this size in arg1 knockout cell line ab281603 (knockout cell lysate ab282955). Faint band remaining in KO sample at same molecular weight is likely to be an isoform of arg1. To generate this image, wild-type and arg1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution
Lane 1 : Myc-DDK tagged Recombinant Human ARG1 protein (full-length, aa 1 to 322) (34.6 KDa)
Lane 2 : Myc-DDK tagged Recombinant Human ARG2 protein (full-length, aa 1 to 354) (36 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Mouse liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab124917 has been referenced in 23 publications.
- Gong B et al. Luteolin activates M2 macrophages and suppresses M1 macrophages by upregulation of hsa_circ_0001326 in THP-1 derived macrophages. Bioengineered 13:5079-5090 (2022). PubMed: 35152837
- Shen LX et al. Talaromyces marneffei Influences Macrophage Polarization and Sterilization Ability via the Arginine Metabolism Pathway in Vitro. Am J Trop Med Hyg 107:600-9 (2022). PubMed: 35895344
- Guo Q et al. SOCS1 Mediates Berberine-Induced Amelioration of Microglial Activated States in N9 Microglia Exposed to β Amyloid. Biomed Res Int 2021:9311855 (2021). PubMed: 34778460
- Li J et al. Lupeol reduces M1 macrophage polarization to attenuate immunologic dissonance and fatty acid deposition in rats with diet-induced metabolic syndrome. Ann Transl Med 9:1534 (2021). PubMed: 34790740
- Li X et al. miR-365 secreted from M2 Macrophage-derived extracellular vesicles promotes pancreatic ductal adenocarcinoma progression through the BTG2/FAK/AKT axis. J Cell Mol Med 25:4671-4683 (2021). PubMed: 33811437