Anti-Liver Carboxylesterase 1 antibody [EP1376Y] (ab53008)

Overview

  • Product nameAnti-Liver Carboxylesterase 1 antibody [EP1376Y]
    See all Liver Carboxylesterase 1 primary antibodies
  • Description
    Rabbit monoclonal [EP1376Y] to Liver Carboxylesterase 1
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Liver Carboxylesterase 1 aa 550 to the C-terminus (C terminal).

  • Positive control
    • U937 cell lysates Human liver tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Associated products

Applications

Our Abpromise guarantee covers the use of ab53008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).
IP 1/100.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionInvolved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
    • Tissue specificityExpressed predominantly in liver with lower levels in heart and lung.
    • Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
    • Post-translational
      modifications
      Contains sialic acid.
      Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.
    • Cellular localizationEndoplasmic reticulum lumen.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAT antibody
      • Acyl coenzyme A cholesterol acyltransferase antibody
      • Acyl-coenzyme A:cholesterol acyltransferase antibody
      • Brain carboxylesterase hBr1 antibody
      • Carboxyesterase ES-3 antibody
      • Carboxylesterase antibody
      • Carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody
      • Carboxylesterase 1 antibody
      • Carboxylesterase 1 deficiency, included antibody
      • Carboxylesterase 2, formerly antibody
      • CE 1 antibody
      • CEH antibody
      • Ces-1 antibody
      • CES1 antibody
      • CES2 antibody
      • CESDD1 antibody
      • Cholesterol ester hydrolase, neutral, macrophage-derived antibody
      • Cholesteryl ester hydrolase antibody
      • Cocaine carboxylesterase antibody
      • EC 3.1.1.1 antibody
      • Egasyn antibody
      • ES-HTEL antibody
      • ES-x antibody
      • Es22 antibody
      • EST1_HUMAN antibody
      • Esterase 22 antibody
      • Esterase antibody
      • hCE 1 antibody
      • HMSE antibody
      • HMSE1 antibody
      • Liver carboxylesterase 1 antibody
      • Liver carboxylesterase 3 antibody
      • Methylumbelliferyl acetate deacetylase 1 antibody
      • MGC117365 antibody
      • MGC156521 antibody
      • Monocyte carboxylesterase deficiency, included antibody
      • Monocyte esterase deficiency, included antibody
      • Monocyte/macrophage serine esterase antibody
      • PCE-1 antibody
      • pI 5.5 esterase antibody
      • Proline-beta-naphthylamidase antibody
      • REH antibody
      • Retinyl ester hydrolase antibody
      • Serine esterase 1 antibody
      • Ses-1 antibody
      • SES1 antibody
      • TGH antibody
      • Triacylglycerol hydrolase antibody
      see all

    Anti-Liver Carboxylesterase 1 antibody [EP1376Y] images

    • Anti-Liver Carboxylesterase 1 antibody [EP1376Y] (ab53008) at 1/10000 dilution + U937 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 65 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • ab53008, at a 1/250 dilution, staining Human SOAT 1 in Liver using Immunohistochemistry, Paraffin embedded tissue.

    References for Anti-Liver Carboxylesterase 1 antibody [EP1376Y] (ab53008)

    ab53008 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. These two antibodies should work equally well in IHC for Human. ab53008 has not successfully been tested for other species. They do have differenct immunogenic peptides but that doesn't mean one should work better than the ...

    Read More

    Thank you for your reply.

    Our ab39327 is actually capable of detecting both the native a processed form of the protein. I've attached the WB image and here is the caption:

    Human ACAT-1 has 550 amino acids with an estimated MW of 65 ...

    Read More

    Thank you for contacting us.

    The MW of SOAT1 according to SwissProt is 64.7 kDa and it is 550 kDa long.

    http://www.uniprot.org/uniprot/P35610

    There may be a processed form of the protein which other antibodies can detect...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"