Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) (ab94047)

Overview

  • Product nameLiver Carboxylesterase 1 overexpression 293T lysate (whole cell)
  • General notesab94047 is a 293T cell transfected lysate in which Human Liver Carboxylesterase 1 has been transiently over-expressed using a pCMV-Liver Carboxylesterase 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. PTM: Contains sialic acid. Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. Similarity: Belongs to the type-B carboxylesterase/lipase family. Tissue specificity: Expressed predominantly in liver with lower levels in heart and lung.

Applications

Our Abpromise guarantee covers the use of ab94047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) images

  • ab94047 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Liver Carboxylesterase 1 antibody (ab89779) at 1/500 dilution

    Lane 1 : Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) (ab94047)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) (ab94047)

ab94047 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"