Anti-liver FABP antibody [2G4] (ab82157)

Overview

  • Product name
    Anti-liver FABP antibody [2G4]
    See all liver FABP primary antibodies
  • Description
    Mouse monoclonal [2G4] to liver FABP
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human liver FABP (aa1-127) purified from E. coli (NP_001434)

  • Positive control
    • HepG2 cell lysates and Human colon cancer
  • General notes
    Isotype: Mouse IgG1 heavy chain and kappa light chain

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    2G4
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/300. for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer.
WB 1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ELISA Use at an assay dependent concentration.

Target

  • Function
    Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
  • Sequence similarities
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain
    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FABP 1 antibody
    • FABP1 antibody
    • FABPL antibody
    • FABPL_HUMAN antibody
    • Fatty Acid Binding Protein 1 antibody
    • Fatty acid binding protein 1 liver antibody
    • Fatty Acid Binding Protein antibody
    • Fatty acid-binding protein 1 antibody
    • Fatty acid-binding protein antibody
    • Fatty acid-binding protein liver antibody
    • L FABP antibody
    • L-FABP antibody
    • liver antibody
    • Liver-type fatty acid-binding protein antibody
    see all

Anti-liver FABP antibody [2G4] images

  • Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    Secondary
    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • ab82157 at 1/100 dilution staining liver FABP in human colon cancer by Immunohistochemistry, Paraffin-embedded tissue detected using Diaminobenzidine (DAB).
  • Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-liver FABP antibody [2G4] (ab82157)

ab82157 has not yet been referenced specifically in any publications.

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Unfortunately, we offer the antibody only in the size listed on the datasheet (100 ul). Sample sizes cannot be offered due to operational reasons. For larger sizes or bulk orders, please contact mailto:sales@abca...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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