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Rabbit polyclonal to liver FABP
Predicted to work with:
Mouse, Rat, Pig, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human liver FABP.
(Peptide available as
This antibody gave a positive signal in WB on human liver tissue lysate. ICC/IF: MCF7 cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Information by UniProt
FABP 1 antibody
Western blot - liver FABP antibody (ab76812)
Anti-liver FABP antibody (ab76812) at 1 µg/ml + Human liver tissue lysate - total protein (
) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
Additional bands at :
160 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
Immunocytochemistry/ Immunofluorescence - liver FABP antibody (ab76812)
ICC/IF image of ab76812 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76812, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-liver FABP antibody (ab76812)
Anti-liver FABP antibody (ab76812) at 1 µg/ml +
Recombinant Human liver FABP protein ( ab82994) at 0.01 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Exposure time : 8 minutes
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"