Overview

  • Product nameAnti-liver FABP antibody [L2B10]
    See all liver FABP primary antibodies
  • Description
    Mouse monoclonal [L2B10] to liver FABP
  • SpecificityThis monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP.
  • Tested applicationsSuitable for: Flow Cyt, Functional Studies, IHC-P, WB, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, African Green Monkey

Properties

Applications

Our Abpromise guarantee covers the use of ab7366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Functional Studies Use at an assay dependent concentration. Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37°C, 5% CO2. * T=24: measure L-FABP in supernatant.
IHC-P Use at an assay dependent concentration.
WB 1/500. PubMed: 18779054
IHC-Fr Use at an assay dependent concentration.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionBinds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
    • Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
    • DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • FABP 1 antibody
      • FABP1 antibody
      • FABPL antibody
      • FABPL_HUMAN antibody
      • Fatty Acid Binding Protein 1 antibody
      • Fatty acid binding protein 1 liver antibody
      • Fatty Acid Binding Protein antibody
      • Fatty acid-binding protein 1 antibody
      • Fatty acid-binding protein antibody
      • Fatty acid-binding protein liver antibody
      • L FABP antibody
      • L-FABP antibody
      • liver antibody
      • Liver-type fatty acid-binding protein antibody
      see all

    Anti-liver FABP antibody [L2B10] images

    • Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.

    • Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.

    • Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-liver FABP antibody [L2B10] (ab7366)

    This product has been referenced in:
    • Smathers RL  et al. Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver. J Lipid Res 54:1335-45 (2013). WB ; Mouse . Read more (PubMed: 23359610) »
    • D'souza AM  et al. Consumption of a high-fat diet rapidly exacerbates the development of fatty liver disease that occurs with chronically elevated glucocorticoids. Am J Physiol Gastrointest Liver Physiol 302:G850-63 (2012). WB ; Rat . Read more (PubMed: 22268100) »

    See all 7 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Cow Tissue lysate - whole (liver tissue lysate)
    Loading amount 10 µg
    Specification liver tissue lysate
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 10 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample African Green Monkey Cell (hepatocyte)
    Specification hepatocyte
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 29 2008

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (hepatocyte)
    Specification hepatocyte
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 29 2008

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (hepatocytes)
    Specification hepatocytes
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% triton x-100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 20 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (cos-7)
    Loading amount 75 µg
    Specification cos-7
    Gel Running Conditions Reduced Denaturing (15% gel)
    Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 18 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (hepatocytes)
    Loading amount 50 µg
    Specification hepatocytes
    Gel Running Conditions Reduced Non-Denaturing (Native)
    Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 18 2008

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (primary hepatocytes)
    Specification primary hepatocytes
    Fixative Methanol
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    Submitted Nov 21 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (liver)
    Loading amount 25 µg
    Specification liver
    Gel Running Conditions Non-reduced Denaturing
    Blocking step Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 07 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Primary Hepatocytes)
    Loading amount 25 µg
    Specification Primary Hepatocytes
    Gel Running Conditions Non-reduced Denaturing
    Blocking step Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 25 2007

    Application Western blot
    Sample Rat Tissue lysate - whole (liver)
    Loading amount 50 µg
    Specification liver
    Gel Running Conditions Non-reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mrs. flower little

    Verified customer

    Submitted Oct 12 2007

    1-10 of 11 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"