Overview

  • Product nameAnti-Livin antibody
    See all Livin primary antibodies
  • Description
    Goat polyclonal to Livin
  • SpecificityExpected to recognise both reported isoforms (as represented by NP_647478.1 and NP_071444.1)
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    RAPVRSRVRT

    from the C-terminal region of Human Livin (common to both isoform NP_647478.1 and NP_071444.1).

  • Positive control
    • Lysate from Human acute lymphoblastic leukaemia cell line MOLT4

Properties

Applications

Our Abpromise guarantee covers the use of ab77190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/4000.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 33 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProtects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.
    • Tissue specificityIsoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.
    • Sequence similaritiesBelongs to the IAP family.
      Contains 1 BIR repeat.
      Contains 1 RING-type zinc finger.
    • Cellular localizationNucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Baculoviral IAP repeat containing 7 antibody
      • Baculoviral IAP repeat containing protein 7 antibody
      • Baculoviral IAP repeat-containing protein 7 antibody
      • BIRC 7 antibody
      • birc7 antibody
      • BIRC7_HUMAN antibody
      • KIAP antibody
      • Kidney inhibitor of apoptosis protein antibody
      • Livin antibody
      • Livin inhibitor of apotosis antibody
      • Melanoma inhibitor of apoptosis protein antibody
      • ML IAP antibody
      • ML-IAP antibody
      • MLIAP antibody
      • RING finger protein 50 antibody
      • RNF 50 antibody
      • RNF50 antibody
      see all

    Anti-Livin antibody images

    • Anti-Livin antibody (ab77190) at 0.3 µg/ml + MOLT4 lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 33 kDa
      Observed band size : 30,33 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 hour

    References for Anti-Livin antibody (ab77190)

    ab77190 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab77190.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"