Overview

  • Product name
  • Description
    Rabbit polyclonal to Livin
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-211 of Human Livin (AAH14475).

  • Positive control
    • WB: A431, H1299, HeLa and HepG2 cell lysates IHC-P: Cal27 xenograft ICc/IF: Hela cell

Properties

Applications

Our Abpromise guarantee covers the use of ab97350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.
  • Tissue specificity
    Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the IAP family.
    Contains 1 BIR repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Nucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Baculoviral IAP repeat containing 7 antibody
    • Baculoviral IAP repeat containing protein 7 antibody
    • Baculoviral IAP repeat-containing protein 7 antibody
    • BIRC 7 antibody
    • birc7 antibody
    • BIRC7_HUMAN antibody
    • KIAP antibody
    • Kidney inhibitor of apoptosis protein antibody
    • Livin antibody
    • Livin inhibitor of apotosis antibody
    • Melanoma inhibitor of apoptosis protein antibody
    • ML IAP antibody
    • ML-IAP antibody
    • MLIAP antibody
    • RING finger protein 50 antibody
    • RNF 50 antibody
    • RNF50 antibody
    see all

Images

  • Anti-Livin antibody (ab97350) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 33 kDa
  • ab97350, at a 1/200 dilution, staining Livin in paraformaldehyde fixed HeLa cell by immunofluorescence.
    The lower image shows ab97350 merged with DNA probe.
  • ab97350, at a 1/100 dilution, staining Livin in paraffin embedded Cal27 xenograft by Immunohistochemistry.

References

This product has been referenced in:
  • Saladi SV  et al. BRG1 promotes survival of UV-irradiated melanoma cells by cooperating with MITF to activate the melanoma inhibitor of apoptosis gene. Pigment Cell Melanoma Res 26:377-91 (2013). WB . Read more (PubMed: 23480510) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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