Overview

  • Product nameAnti-LKB1 antibody [Ley 37D/G6]
    See all LKB1 primary antibodies
  • Description
    Mouse monoclonal [Ley 37D/G6] to LKB1
  • SpecificityThis antibody detects a single clean band representing LKB1/STK11 in Western blots on cells expressing LKB1.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to LKB1.

  • Positive control
    • Adenocarcinoma H441 cell line, Lung carcinoma cell line H1299

Properties

Applications

Our Abpromise guarantee covers the use of ab15095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 48.6 kDa).

Target

  • FunctionEssential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
  • Tissue specificityUbiquitously expressed. Strongest expression in testis and fetal liver.
  • Involvement in diseaseDefects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by a cAMP-dependent protein kinase.
  • Cellular localizationNucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLKB1 antibody
    • Liver kinase B1 antibody
    • LKB1 antibody
    • PJS antibody
    • Polarization related protein LKB1 antibody
    • Renal carcinoma antigen NY-REN-19 antibody
    • Serine/Threonine Kinase 11 antibody
    • Serine/threonine protein kinase 11 antibody
    • Serine/threonine protein kinase LKB1 antibody
    • Serine/threonine protein kinase STK11 antibody
    • Serine/threonine-protein kinase 11 antibody
    • Serine/threonine-protein kinase LKB1 antibody
    • Serine/threonine-protein kinase XEEK1 antibody
    • Stk11 antibody
    • STK11_HUMAN antibody
    see all

Anti-LKB1 antibody [Ley 37D/G6] images



  • Predicted band size : 48.6 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: LKB1 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab15095 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab15095 was shown to recognize LKB1 when LKB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. ab15095 and ab181602 (loading control to LKB1) were diluted 1/100 and 1/10 000 and incubated overnight at 4°C. Blots were developed withGoat anti-Mouse IgG H&L (IRDye® 800CW) ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-LKB1 antibody [Ley 37D/G6] (ab15095) at 1/1000 dilution

    Lane 1 : As above
    Lane 2 : As above

    Secondary
    Polyclonal goat anti-mouse conjugated with horse radish peroxidase.

    Predicted band size : 48.6 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Negative control (Lane 1): LKB1 knockout MEF cells.

    Positive control (Lane 2): wildtype MEF cells.

    Blocking step: 5% milk as blocking agent for 1 hour at 25 °C.

    Incubation: 16 hours at 4 °C, using 5% milk in TBS/0.1 Tween as diluent.

     

  • ab15095 staining LKB1 in human hepatocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Citrate buffer ph 6 for 20 minutes at 97ºC. Samples were incubated with primary antibody (1/100 in diluent) for 1 hour at 37°C. A HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-LKB1 antibody [Ley 37D/G6] (ab15095)

This product has been referenced in:
  • Houde VP  et al. Investigation of LKB1 Ser431 phosphorylation and Cys433 farnesylation using mouse knockin analysis reveals an unexpected role of prenylation in regulating AMPK activity. Biochem J 458:41-56 (2014). Read more (PubMed: 24295069) »
  • Werle K  et al. Liver kinase B1 regulates the centrosome via PLK1. Cell Death Dis 5:e1157 (2014). Read more (PubMed: 24722282) »

See all 16 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Hepatocarcinoma (HCC))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6, 20 min at 97ºC IN A PT LINK
Permeabilization No
Specification Hepatocarcinoma (HCC)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Miss. Virginia Gutiérrez

Verified customer

Submitted Jan 07 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (MEF)
Specification MEF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2013

Thank you for contacting us.

I have performed a sequence alignment for LKB1 for Dictyostelium and for human (which is used to raise most of our antibodies.) Unfortunately there is only a total of 33% homology between the two proteins. I have...

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I have found the amino acid sequence for LKB1 for Dictyostelium which can be found at http://www.uniprot.org/uniprot/Q15831.fasta

I have contacted the lab to see if any of our antibodies would be compat...

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I had a quick chat with my colleague in lab. They have confirmed that the antibody has been raised against the full length protein so they do not have determined the epitope this antibody is recognizing

I ...

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I have checked with supplying the lab, but unfortunately, the immunogen is not available for sale.

Alternatively, I could recommend an isotype control (mouse IgG2b) to be used as negative control, for i...

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Thank you for contacting us.

The immunogen was ahighly purified recombinant full length protein made in E. coli, but is not a peptide. I will check with the lab if the immunogen might be commercially available and let you know what I find ou...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (neurons)
Loading amount 50 µg
Specification neurons
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (neurons)
Specification neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton-x-100
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

Submitted Jan 31 2011

Application ChIP
Sample Human Cell lysate - nuclear (cervical tumor cell lines)
Specification cervical tumor cell lines
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Semiquantitative PCR
Positive control HeLa and SiHa cells
Negative control primary keratinocytes
Username

Abcam user community

Verified customer

Submitted Jan 10 2011

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"