Anti-LKB1 (phospho T189) antibody (ab60987)

Overview

  • Product name
    Anti-LKB1 (phospho T189) antibody
    See all LKB1 primary antibodies
  • Description
    Rabbit polyclonal to LKB1 (phospho T189)
  • Specificity
    Detects endogenous levels of LKB1 only when phosphorylated at threonine 189.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of throsine 189 (G-G-TP-L-K).

Properties

Applications

Our Abpromise guarantee covers the use of ab60987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).

Target

  • Function
    Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
  • Tissue specificity
    Ubiquitously expressed. Strongest expression in testis and fetal liver.
  • Involvement in disease
    Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by a cAMP-dependent protein kinase.
  • Cellular localization
    Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLKB1 antibody
    • Liver kinase B1 antibody
    • LKB1 antibody
    • PJS antibody
    • Polarization related protein LKB1 antibody
    • Renal carcinoma antigen NY-REN-19 antibody
    • Serine/Threonine Kinase 11 antibody
    • Serine/threonine protein kinase 11 antibody
    • Serine/threonine protein kinase LKB1 antibody
    • Serine/threonine protein kinase STK11 antibody
    • Serine/threonine-protein kinase 11 antibody
    • Serine/threonine-protein kinase LKB1 antibody
    • Serine/threonine-protein kinase XEEK1 antibody
    • Stk11 antibody
    • STK11_HUMAN antibody
    see all

Anti-LKB1 (phospho T189) antibody images

  • All lanes : Anti-LKB1 (phospho T189) antibody (ab60987) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells, treated with PMA (125ng/ml, 30mins).
    Lane 2 : Extracts from NIH/3T3 cells, treated with PMA (125ng/ml, 30mins) with immunizing phosphopeptide


    Predicted band size : 48 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-LKB1 (phospho T189) antibody (ab60987)

ab60987 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The link to the database entry for the human LKB1 protein is:

http://www.uniprot.org/uniprot/Q15831

I hope this information is helpful to you. Please do not hesitate to contact us if you need...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount
50 µg
Specification
Mouse embryonic fibroblast
Gel Running Conditions
Reduced Denaturing (10-20% tris-tricine)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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