The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
FunctionCortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.
Tissue specificityExpressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease.
Sequence similaritiesBelongs to the WD repeat L(2)GL family. Contains 14 WD repeats.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated at least at Ser-663 by PRKCI.
Cellular localizationCytoplasm > cytoskeleton. Localized to the lateral membrane during the polarization and formation cell-cell contacts.