The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionPart of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the initial phase of the establishment of epithelial cell polarity.
Sequence similaritiesBelongs to the WD repeat L(2)GL family. Contains 14 WD repeats.
Post-translational modificationsPhosphorylated at Ser-653 by PRKCI. Phosphorylation is enhanced during cell polarization induced by calcium. Phosphorylation may occur during the cell-cell contact-induced cell polarization and may contribute to the segregation of LLGL2 from the PRKCI/aPKC and PARD6B/Par-6 complex.
Cellular localizationCytoplasm. Localized in the perinuclear structure and faintly at the cell-cell contacts sites in the interphase. Localized at the cell periphery during metaphase. Cortical localization in mitotic cells. Found in the lateral region of polarized epithelial cells.
LLGL2 antibody (ab55423) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human salivary gland.
Western blot - LLGL2 antibody (ab55423)
Western blot against tagged recombinant protein immunogen using ab55423 LLGL2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
Flow Cytometry-Anti-LLGL2 antibody(ab55423)
Overlay histogram showing K562 cells stained with ab55423 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55423, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-LLGL2 antibody (ab55423)
This product has been referenced in:
Linch M et al. A cancer-associated mutation in atypical protein kinase C? occurs in a substrate-specific recruitment motif. Sci Signal6:ra82 (2013).
Read more (PubMed: 24045153) »
Sripathy S et al. Mammalian Llgl2 Is Necessary for Proper Branching Morphogenesis during Placental Development. Mol Cell Biol31:2920-33 (2011).
Read more (PubMed: 21606200) »