Overview

  • Product name
  • Description
    Rabbit polyclonal to LMCD1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 160-293 of Human LMCD1.

  • Positive control
    • Human cerebellum tissue; RT-4, U-251 MG cell lysate; Human Liver and Tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
  • Tissue specificity
    Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
  • Sequence similarities
    Contains 2 LIM zinc-binding domains.
    Contains 1 PET domain.
  • Domain
    The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
  • Cellular localization
    Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dyxin antibody
    • LIM and cysteine rich domains 1 antibody
    • LIM and cysteine rich domains protein 1 antibody
    • LIM and cysteine-rich domains protein 1 antibody
    • LMCD1 antibody
    • LMCD1_HUMAN antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cell junctions. Recommended concentration of ab121788 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-LMCD1 antibody (ab121788) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121788, at 1/400 dilution, staining LMCD1 in paraffin-embedded Human cerebellum tissue, by Immunohistochemistry.

References

ab121788 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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