The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionTranscriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
Tissue specificityExpressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
Sequence similaritiesContains 2 LIM zinc-binding domains. Contains 1 PET domain.
DomainThe LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
Cellular localizationCytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.