Recombinant
RabMAb

Anti-LMCD1 antibody [EPR11443(B)] (ab169760)

Overview

  • Product name
    Anti-LMCD1 antibody [EPR11443(B)]
    See all LMCD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11443(B)] to LMCD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LMCD1. The exact sequence is proprietary.
    Database link: Q9NZU5

  • Positive control
    • Human skeletal muscle and SH-SY5Y lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
    • Tissue specificity
      Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
    • Sequence similarities
      Contains 2 LIM zinc-binding domains.
      Contains 1 PET domain.
    • Domain
      The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
    • Cellular localization
      Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dyxin antibody
      • LIM and cysteine rich domains 1 antibody
      • LIM and cysteine rich domains protein 1 antibody
      • LIM and cysteine-rich domains protein 1 antibody
      • LMCD1 antibody
      • LMCD1_HUMAN antibody
      see all

    Images

    • Detection of LMCD1  by Western Blot of Immunprecipitate.
      Human skeletal muscle lysate immunoprecipitated using ab169760 at 1/10 dilution (lane 1) or a 1 X PBS negative control (lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • All lanes : Anti-LMCD1 antibody [EPR11443(B)] (ab169760) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    References

    ab169760 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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