The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
1/10 - 1/100.
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
Contains 2 LIM zinc-binding domains. Contains 1 PET domain.
The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
Detection of LMCD1 by Western Blot of Immunprecipitate. Human skeletal muscle lysate immunoprecipitated using ab169760 at 1/10 dilution (lane 1) or a 1 X PBS negative control (lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Western blot - Anti-LMCD1 [EPR11443(B)] antibody (ab169760)
All lanes : Anti-LMCD1 antibody [EPR11443(B)] (ab169760) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate Lane 2 : SH-SY5Y lysate