Recombinant Anti-LMCD1 antibody [EPR11444] (ab179454)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11444] to LMCD1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LMCD1 antibody [EPR11444]
See all LMCD1 primary antibodies -
Description
Rabbit monoclonal [EPR11444] to LMCD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human LMCD1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NZU5 -
Positive control
- WB: Human skeletal muscle lysate. ICC/IF: MCF7 cells. IP: LMCD1 IP in human skeletal muscle lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11444 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
Target
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Function
Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway. -
Tissue specificity
Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle. -
Sequence similarities
Contains 2 LIM zinc-binding domains.
Contains 1 PET domain. -
Domain
The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6. -
Cellular localization
Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29995 Human
- Omim: 604859 Human
- SwissProt: Q9NZU5 Human
- Unigene: 475353 Human
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Alternative names
- Dyxin antibody
- LIM and cysteine rich domains 1 antibody
- LIM and cysteine rich domains protein 1 antibody
see all
Images
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Anti-LMCD1 antibody [EPR11444] (ab179454) at 1/1000 dilution + Human skeletal muscle lysate at 10 µg
Predicted band size: 41 kDa -
Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling LMCD1 with ab179454 at 1/100 dilution.
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Western blot analysis on immunoprecipitation pellet from (Lane 1) Human skeletal muscle lysate or (Lane 2) 1X PBS (negative control) using ab179454, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab179454 has been referenced in 3 publications.
- Govatati S et al. Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions. Arterioscler Thromb Vasc Biol 40:1256-1274 (2020). PubMed: 32160773
- Govatati S et al. NFATc1-E2F1-LMCD1-Mediated IL-33 Expression by Thrombin Is Required for Injury-Induced Neointima Formation. Arterioscler Thromb Vasc Biol 39:1212-1226 (2019). PubMed: 31043075
- Janjanam J et al. LIM and cysteine-rich domains 1 is required for thrombin-induced smooth muscle cell proliferation and promotes atherogenesis. J Biol Chem 293:3088-3103 (2018). PubMed: 29326163