Recombinant
RabMAb

Anti-LMCD1 antibody [EPR11444] (ab179454)

Overview

  • Product name
    Anti-LMCD1 antibody [EPR11444]
    See all LMCD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11444] to LMCD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LMCD1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NZU5

  • Positive control
    • Human skeletal muscle lysate; MCF7 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
    • Tissue specificity
      Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
    • Sequence similarities
      Contains 2 LIM zinc-binding domains.
      Contains 1 PET domain.
    • Domain
      The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
    • Cellular localization
      Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dyxin antibody
      • LIM and cysteine rich domains 1 antibody
      • LIM and cysteine rich domains protein 1 antibody
      • LIM and cysteine-rich domains protein 1 antibody
      • LMCD1 antibody
      • LMCD1_HUMAN antibody
      see all

    Images

    • Immunofluorescent analysis of MCF7 cells labeling LMCD1 with ab179454 at 1/100 dilution.

    • Anti-LMCD1 antibody [EPR11444] (ab179454) at 1/1000 dilution + Human skeletal muscle lysate at 10 µg

      Predicted band size: 41 kDa

    • Western blot analysis on immunoprecipitation pellet from (1) Human skeletal muscle lysate or (2) 1XPBS (negative control) using ab179454, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab179454 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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